BMRB: 7314

The backbone chemical shifts of ribosomal protein L11 in the complex with rRNA

Authors

Lee, D., Wang, Y.

Assembly

L11 in complex with rRNA

Entity

1. L11 (polymer, Thiol state: all free), 147 monomers, 15504.93 Da Detail

MKKVVAVVKL QLPAGKATPA PPVGPALGQH GANIMEFVKA FNAATANMGD AIVPVEITIY ADRSFTFVTK TPPASYLIRK AAGLEKGAHK PGREKVGRIT WEQVLEIAKQ KMPDLNTTDL EAAARMIAGS ARSMGVEVVG APEVKDA

2. RNA (polymer, Thiol state: not present), 58 monomers, 18765.12 Da Detail

GCCAGGAUGU UGGCUUAGAA GCAGCCAUCA UUUAAAGAAA GCGUAAUAGC UCACUGGU

Total weight

34270.047 Da

Max. entity weight

18765.12 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS, RG refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 70.7 %, Completeness: 32.0 %, Completeness (bb): 38.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L) polyribonucleotide

	Total	1H	13C	15N
All	32.0 % (822 of 2570)	21.6 % (292 of 1354)	37.7 % (393 of 1042)	78.7 % (137 of 174)
Backbone	38.6 % (578 of 1498)	23.2 % (149 of 643)	40.9 % (294 of 719)	99.3 % (135 of 136)
Sidechain	30.3 % (366 of 1207)	20.1 % (143 of 711)	48.3 % (221 of 458)	5.3 % (2 of 38)
Aromatic	12.8 % (43 of 336)	13.1 % (22 of 168)	14.5 % (20 of 138)	3.3 % (1 of 30)
Methyl	82.6 % (157 of 190)	80.0 % (76 of 95)	85.3 % (81 of 95)

1. L11

MKKVVAVVKL QLPAGKATPA PPVGPALGQH GANIMEFVKA FNAATANMGD AIVPVEITIY ADRSFTFVTK TPPASYLIRK AAGLEKGAHK PGREKVGRIT WEQVLEIAKQ KMPDLNTTDL EAAARMIAGS ARSMGVEVVG APEVKDA

2. RNA

GCCAGGAUGU UGGCUUAGAA GCAGCCAUCA UUUAAAGAAA GCGUAAUAGC UCACUGGU

Show sample condition

Sample

Temperature 308 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	L11	[U-13C; U-15N; U-2H]		0.7 mM
2	RNA			0.0 ~ 0.0 mM

Hide sample codition

LACS Plot; CA

Referencing offset: 0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	L11	protons	0.01 ppm	internal	indirect	0.0

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	L11	protons	0.01 ppm	internal	indirect	0.0

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2E34, Strand ID: A Detail

---

Release date

2007-05-03

Citation

The structure of free L11 and functional dynamics of L11 in free, L11-rRNA(58 nt) binary and L11-rRNA(58 nt)-thiostrepton ternary complexes
Lee, D., Walsh, J., Yu, P., Markus, M., Choli-Papadopoulou, T., Schwieters, C., Krueger, S., Draper, D., Wang, Y.
J. Mol. Biol. (2007), 367, 1007-1022, PubMed 17292917 , DOI 10.1016/j.jmb.2007.01.013 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. L11, : 1 : 33 : 11 : 282 entities Detail

Related entities 2. RNA, : 100 : 6 entities Detail

Interaction partners 1. L11, : 1 interactors Detail

Experiments performed 6 experiments Detail