BMRB: 7315

The backbone chemical shifts of ribosomal protein L11 in the complex with rRNA and thiostrepton

Authors

Lee, D., Wang, Y.

Assembly

L11 in complex with rRNA

Entity

1. L11 (polymer, Thiol state: all free), 147 monomers, 15504.93 Da Detail

MKKVVAVVKL QLPAGKATPA PPVGPALGQH GANIMEFVKA FNAATANMGD AIVPVEITIY ADRSFTFVTK TPPASYLIRK AAGLEKGAHK PGREKVGRIT WEQVLEIAKQ KMPDLNTTDL EAAARMIAGS ARSMGVEVVG APEVKDA

2. RNA (polymer, Thiol state: not present), 58 monomers, 18765.12 Da Detail

GCCAGGAUGU UGGCUUAGAA GCAGCCAUCA UUUAAAGAAA GCGUAAUAGC UCACUGGU

3. Thiostrepton antibiotic (polymer), na Da

Total weight

34270.047 Da

Max. entity weight

18765.12 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS, SANS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 69.8 %, Completeness: 22.2 %, Completeness (bb): 36.6 % Detail

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Polymer type: polypeptide(L) polyribonucleotide

	Total	1H	13C	15N
All	22.2 % (571 of 2570)	12.0 % (162 of 1354)	26.3 % (274 of 1042)	77.6 % (135 of 174)
Backbone	36.6 % (548 of 1498)	22.6 % (145 of 643)	37.4 % (269 of 719)	98.5 % (134 of 136)
Sidechain	12.0 % (145 of 1207)	2.5 % (18 of 711)	27.5 % (126 of 458)	2.6 % (1 of 38)
Aromatic	0.6 % (2 of 336)	0.6 % (1 of 168)	0.0 % (0 of 138)	3.3 % (1 of 30)
Methyl	16.8 % (32 of 190)	5.3 % (5 of 95)	28.4 % (27 of 95)

1. L11

MKKVVAVVKL QLPAGKATPA PPVGPALGQH GANIMEFVKA FNAATANMGD AIVPVEITIY ADRSFTFVTK TPPASYLIRK AAGLEKGAHK PGREKVGRIT WEQVLEIAKQ KMPDLNTTDL EAAARMIAGS ARSMGVEVVG APEVKDA

2. RNA

GCCAGGAUGU UGGCUUAGAA GCAGCCAUCA UUUAAAGAAA GCGUAAUAGC UCACUGGU

Show sample condition

Sample

Temperature 308 K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	L11	[U-13C; U-15N; U-2H]		0.7 mM
2	RNA			0.0 ~ 0.0 mM
3	Thiostrepton			0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	L11	protons	0.01 ppm	internal	indirect	0.0

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LACS Plot; CB

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	L11	protons	0.01 ppm	internal	indirect	0.0

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Protein Blocks Logo

Calculated from 1 models in PDB: 2E36, Strand ID: A Detail

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Release date

2007-05-03

Citation

The structure of free L11 and functional dynamics of L11 in free, L11-rRNA(58 nt) binary and L11-rRNA(58 nt)-thiostrepton ternary complexes
Yu, P., Lee, D., Walsh, J., Markus, M., Choli-Papadopoulou, T., Schwieters, C., Krueger, S., Draper, D., Wang, Y.
J. Mol. Biol. (2007), 367, 1007-1022, PubMed 17292917 , DOI 10.1016/j.jmb.2007.01.013 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. L11, : 1 : 33 : 11 : 282 entities Detail

Related entities 2. RNA, : 100 : 6 entities Detail

Interaction partners 1. L11, : 1 interactors Detail

Experiments performed 6 experiments Detail