Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTDL IYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 41.4 % (456 of 1102) | 21.5 % (123 of 573) | 58.3 % (252 of 432) | 83.5 % (81 of 97) |
Backbone | 75.2 % (418 of 556) | 47.6 % (90 of 189) | 89.5 % (248 of 277) | 88.9 % (80 of 90) |
Sidechain | 18.9 % (120 of 635) | 8.9 % (34 of 384) | 34.8 % (85 of 244) | 14.3 % (1 of 7) |
Aromatic | 4.1 % (4 of 98) | 8.2 % (4 of 49) | 0.0 % (0 of 48) | 0.0 % (0 of 1) |
Methyl | 11.7 % (11 of 94) | 8.5 % (4 of 47) | 14.9 % (7 of 47) |
1. Im7*
MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTDL IYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQGTemperature 298 (±0.1) K, pH 7 (±0.2), Details doubly-labelled (13C/15N) Im7* sample for backbone resonance assignments.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Im7* | [U-13C; U-15N] | protein | 1 (±0.2) mM |
2 | potassium phosphate buffer | 50 mM | ||
3 | sodium sulphate | 400 mM | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |
Temperature 298 (±0.1) K, pH 7 (±0.2), Details singly-labelled (15N) Im7* sample for relaxation data measurement.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Im7* | [U-99% 15N] | protein | 1 (±0.2) mM |
7 | potassium phosphate buffer | 50 mM | ||
8 | sodium sulphate | 400 mM | ||
9 | H2O | 90 % | ||
10 | D2O | 10 % |