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Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
Authors
Whittaker, S.B.-M., Spence, G.R., Grossmann, G.J., Radford, S.E., Moore, G.R.
Assembly
Monomer
Entity
1. Monomer (polymer, Thiol state: not present), 94 monomers, 10762.65 Da Detail

MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTDA AYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQG


Formula weight
10762.65 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 78.7 %, Completeness: 29.8 %, Completeness (bb): 58.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All29.8 % (325 of 1090)11.5 % (65 of 565)45.8 % (195 of 426)65.7 % (65 of 99)
Backbone58.1 % (320 of 551)32.6 % (60 of 184)70.4 % (195 of 277)72.2 % (65 of 90)
Sidechain 9.7 % (61 of 628) 1.3 % (5 of 381)23.5 % (56 of 238) 0.0 % (0 of 9)
Aromatic 0.0 % (0 of 98) 0.0 % (0 of 49) 0.0 % (0 of 48) 0.0 % (0 of 1)
Methyl 5.6 % (5 of 90) 0.0 % (0 of 45)11.1 % (5 of 45)

1. L53AI54A

MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTDA AYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQG

Sample #1

Temperature 298 (±0.1) K, pH 7 (±0.2), Details doubly-labelled (13C/15N) Im7*L53AI54A sample for backbone resonance assignments.


#NameIsotope labelingTypeConcentration
1Im7*[U-13C; U-15N]protein1 (±0.2) mM
2potassium phosphate buffer50 mM
3sodium sulphate400 mM
4H2O90 %
5D2O10 %
Sample #2

Temperature 298 (±0.1) K, pH 7 (±0.2), Details singly-labelled (15N) Im7*L53AI54A sample for relaxation data measurement and 15N-edited NOESY experiment.


#NameIsotope labelingTypeConcentration
6Im7*[U-99% 15N]protein1 (±0.2) mM
7potassium phosphate buffer50 mM
8sodium sulphate400 mM
9H2O90 %
10D2O10 %

LACS Plot; CA
Referencing offset: -0.2 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.2 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.21 ppm, Outliers: 2 Detail
Release date
2007-05-03
Citation
NMR analysis of the conformational properties of the trapped on-pathway folding intermediate of the bacterial immunity protein Im7
Whittaker, S.B.-M., Spence, G.R., Grossmann, G.J., Radford, S.E., Moore, G.R.
J. Mol. Biol. (2007), 366, 1001-1015, PubMed 17188712 , DOI 10.1016/j.jmb.2006.11.012 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 7316 released on 2007-05-03
    Title Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
  BMRB: 7318 released on 2007-05-03
    Title Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
Related entities 1. Monomer, : 1 : 12 : 41 entities Detail
Interaction partners 1. Monomer, : 3 interactors Detail
Experiments performed 7 experiments Detail
Keywords Im7, intermediate, NMR, partially folded, Protein folding, SAXS, conformational properties, protein folding