BMRB: 7330

Solution Structure of BmKalphaIT01, an alpha-insect toxin from the Venom of the Chinese Scorpion Buthus martensi Karsch

Authors

Tong, X.T., Chen, X., Wu, H.M.

Assembly

Alpha-neurotoxin TX12

Entity

1. Alpha-neurotoxin TX12 (polymer, Thiol state: all disulfide bound), 64 monomers, 7188.129 Da Detail

VRDAYIAQNY NCVYHCARDA YCNELCTKNG AKSGSCPYLG EHKFACYCKD LPDNVPIRVP GKCH

Formula weight

7188.129 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS12:SG	1:CYS63:SG
2	disulfide	sing	1:CYS16:SG	1:CYS36:SG
3	disulfide	sing	1:CYS22:SG	1:CYS46:SG
4	disulfide	sing	1:CYS26:SG	1:CYS48:SG

Source organism

Mesobuthus martensii

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.2 %, Completeness (bb): 99.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.2 % (372 of 375)	99.2 % (372 of 375)
Backbone	99.2 % (127 of 128)	99.2 % (127 of 128)
Sidechain	99.2 % (245 of 247)	99.2 % (245 of 247)
Aromatic	100.0 % (35 of 35)	100.0 % (35 of 35)
Methyl	100.0 % (25 of 25)	100.0 % (25 of 25)

1. Alpha-neurotoxin TX12

VRDAYIAQNY NCVYHCARDA YCNELCTKNG AKSGSCPYLG EHKFACYCKD LPDNVPIRVP GKCH

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 300 K, pH 4.9, Details 1.4mM BmKalphaIT01; 20mM phosphate buffer; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alpha-neurotoxin_TX12	none		1.4 mM
2	phosphate buffer			20 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 4.9, Details 1.4mM BmKalphaIT01; 20mM phosphate buffer; 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	Alpha-neurotoxin_TX12	none		1.4 mM
6	phosphate buffer			20 mM
7	D2O			100 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E0H, Strand ID: A Detail

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Release date

2007-10-08

Citation

Solution structure of BmKalphaIT01, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch
Tong, X.T., Zhu, J., Ma, Y.G., Chen, X., Wu, G., He, F.H., Cao, C., Wu, H.M.
Biochemistry (2007), 46, 11322-11330, PubMed 17877370 , DOI 10.1021/bi7006788 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Alpha-neurotoxin TX12, : 1 : 2 : 151 entities Detail

Experiments performed 6 experiments Detail