BMRBj - BMREntryMaster

Found 1 Document (1.261 seconds)

BMRB: 7340

4CK0

NMR assignments for a helical diacylglycerol kinase (DAGK), 40 kDa membrane protein from E. coli

Authors

Kim, H., Oxenoid, K., Jacob, J., Sonnichsen, F.D., Sanders, C.R.

Assembly

DAGK homotrimer

Entity

1. DAGK homotrimer (polymer, Thiol state: all free), 122 monomers, 13250.46 × 3 Da Detail

MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDACTRVLLI SSVMLVMIVE LLNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIDAV ITWCILLWSH FG

Total weight

39751.38 Da

Max. entity weight

13250.46 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 79.5 %, Completeness: 33.5 %, Completeness (bb): 60.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.5 % (463 of 1381)	15.7 % (109 of 694)	47.7 % (265 of 555)	67.4 % (89 of 132)
Backbone	60.9 % (446 of 732)	37.7 % (95 of 252)	73.2 % (262 of 358)	73.0 % (89 of 122)
Sidechain	12.6 % (96 of 763)	3.2 % (14 of 442)	26.4 % (82 of 311)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 114)	0.0 % (0 of 57)	0.0 % (0 of 52)	0.0 % (0 of 5)
Methyl	13.4 % (27 of 202)	7.9 % (8 of 101)	18.8 % (19 of 101)

1. DAGK

MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDACTRVLLI SSVMLVMIVE LLNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIDAV ITWCILLWSH FG

Show sample condition

Sample

Temperature 318 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAGK	[U-13C; U-15N; U-2H]		3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

Release date

2006-12-05

Citation

NMR assignments for a helical 40 kDa membrane protein
Oxenoid, K., Kim, H., Jacob, J., Sonnichsen, F., Sanders, C.
J. Am. Chem. Soc. (2004), 126, 5048-5049, PubMed 15099070 , DOI 10.1021/ja049916m , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15019 released on 2006-12-05
Title NMR assignments for a helical diacylglycerol kinase (DAGK), 40 kDa membrane protein from E. coli

Related entities 1. DAGK homotrimer, : 1 : 5 : 23 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7340_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7340_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	424		76.2 (chain: 1, length: 122)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 122, Sequence coverage: 76.2%
- Total number of assignments
  - ¹H chemical shifts: 86
  - ¹³C chemical shifts: 252
  - ¹⁵N chemical shifts: 86
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	105	0	0.0
¹³C chemical shifts	105	16	15.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	694	86	12.4
¹³C chemical shifts	555	252	45.4
¹⁵N chemical shifts	138	86	62.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	252	86	34.1
¹³C chemical shifts	244	176	72.1
¹⁵N chemical shifts	122	86	70.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	442	0	0.0
¹³C chemical shifts	311	76	24.4
¹⁵N chemical shifts	16	0	0.0

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Found 1 Document (1.261 seconds)

BMRB: 7340

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. DAGK homotrimer, : 1 : 5 : 23 entities Detail

Experiments performed 5 experiments Detail

Instrument

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Chemical shift validation 3 contents Detail