BMRB: 7386

Engrailed homeodomain helix-turn-helix motif

Authors

Religa, T.L.

Assembly

homeobox

Entity

1. homeobox (polymer, Thiol state: not present), 44 monomers, 5407.112 Da Detail

AKREFNENRY LTERRRQQLS SELGLNEAQI KIWFQNKRAK IKKS

Formula weight

5407.112 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, coupling_constants, RDCs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.7 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.7 % (506 of 577)	94.6 % (296 of 313)	78.2 % (165 of 211)	84.9 % (45 of 53)
Backbone	98.5 % (260 of 264)	96.6 % (86 of 89)	100.0 % (131 of 131)	97.7 % (43 of 44)
Sidechain	81.2 % (289 of 356)	93.8 % (210 of 224)	62.6 % (77 of 123)	22.2 % (2 of 9)
Aromatic	50.0 % (20 of 40)	95.0 % (19 of 20)	0.0 % (0 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)

1. Segmentation polarity homeobox protein engrailed

AKREFNENRY LTERRRQQLS SELGLNEAQI KIWFQNKRAK IKKS

Show sample condition

Sample

Solvent system 93%H2O / 7% D2O, Pressure 1 atm, Temperature 278 K, pH 5.7, Details 500uM engrailed fragment 16-59, 50mM d-acetate, 100 mM NaCl, pH 5.7, 93%H2O / 7% D2O

#	Name	Isotope labeling	Type	Concentration
1	homeobox	natural abundance		500 uM
2	d-acetate			50 mM
3	NaCl			100 mM
4	H2O			93 %
5	D2O			7 %

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Calculated from 25 models in PDB: 2P81, Strand ID: A Detail

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Coupling constant

86 J values in 1 lists
Pressure 1 atm, Temperature 278 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 278 K, pH 5.7

Experiment name 2D NOESY, 2D TOCSY

List #1 coupling_constant_list_1

RDC

43 RDC values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 278 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 278 K, pH 5.7

Experiment name DSSE

List #1 RDC_list_1, RDC code 1DHNN, Field strength (¹H) 500 MHz

Release date

2007-10-28

Citation

The helix-turn-helix motif as an ultrafast independently folding domain: the pathway of folding of Engrailed homeodomain
Religa, T.L., Johnson, C.M., Vu, D.M., Brewer, S.H., Dyer, R.B., Fersht, A.R.
Proc. Natl. Acad. Sci. U. S. A. (2007), 104, 9272-9277, PubMed 17517666 , DOI 10.1073/pnas.0703434104 , Abstract

Related entities 1. homeobox, : 1 : 1 : 9 : 286 entities Detail

Interaction partners 1. homeobox, : 10 interactors Detail

Experiments performed 2 experiments Detail