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Found 1 Document (1.383 seconds)

BMRB: 7391

3BAS

Electrostatic contributions to the stability of the GCN4 leucine zipper structure.

Authors

Matousek, W.M., Alexandrescu, A.T.

Assembly

GCN4

Entity

1. GCN4 (polymer, Thiol state: not reported), 33 monomers, 3828.347 Da Detail

GSMKQLEDKV EELLSKNYHL ENEVARLKKL VGE

Formula weight

3828.347 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 51.4 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.4 % (207 of 403)	36.9 % (79 of 214)	62.7 % (96 of 153)	88.9 % (32 of 36)
Backbone	95.5 % (189 of 198)	92.6 % (63 of 68)	96.9 % (94 of 97)	97.0 % (32 of 33)
Sidechain	20.3 % (48 of 236)	11.0 % (16 of 146)	36.8 % (32 of 87)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 12)	0.0 % (0 of 6)	0.0 % (0 of 6)
Methyl	5.3 % (2 of 38)	0.0 % (0 of 19)	10.5 % (2 of 19)

1. GCN4p-wt

GSMKQLEDKV EELLSKNYHL ENEVARLKKL VGE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Release date

2009-04-14

Citation

Electrostatic contributions to the stability of the GCN4 leucine zipper structure
Matousek, W.M., Ciani, B., Fitch, C.A., Garcia-Moreno, B.E., Kammerer, R.A., Alexandrescu, A.T.
J. Mol. Biol. (2007), 374, 206-219, PubMed 17920624 , DOI 10.1016/j.jmb.2007.09.007 , Abstract

Related entities 1. GCN4, : 1 : 5 : 58 : 75 entities Detail

Interaction partners 1. GCN4, : 13 interactors Detail

More details for 13 interactors identified by 9 citations

Experiments performed 6 experiments Detail

1 3D HNCACB

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

2 3D HNCA

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

3 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

4 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

5 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

6 3D HNCO

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 2.3, Details 1.8 mM 13C/15N GCN4p-wt, 6 M urea, 150 mM NaCl, 10 mM phosphate buffer, 10% D2O, pH 2.3, 25C

#	Name	Isotope labeling	Type	Concentration
1	GCN4p-wt	[U-13C; U-15N]		1.8 mM
2	urea	natural abundance		6 M
3	sodium chloride	natural abundance		150 mM
4	sodium phosphate	natural abundance		10 mM
5	D2O	natural abundance		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7391_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7391_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	denatured_chem_shift_list_1	Warning	188		100.0 (chain: 1, length: 33)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: denatured_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 33, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 94
  - ¹H chemical shifts: 62
  - ¹⁵N chemical shifts: 32
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1