Pseudo Contact shifts and RDCs of Galectin-3 with C-terminal LBT tagged at a proton frequency of 600MHz.
MLIVPYNLPL PGGVVPRMLI TILGTVKPNA NRIALDFQRG NDVAFHFNPR FNERNRRVIV CNTKLDNNWG REERQSVFPF ESGKPFKIQV LVEPDHFKVA VNDAHLLQYN HRVKKLNEIS KLGISGDIDL TSASYTMIYI DTNNDGWYEG DELLA
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 4.8 % (47 of 970) | 4.8 % (47 of 970) |
Backbone | 11.9 % (37 of 311) | 11.9 % (37 of 311) |
Sidechain | 1.5 % (10 of 659) | 1.5 % (10 of 659) |
Aromatic | 0.0 % (0 of 80) | 0.0 % (0 of 80) |
Methyl | 2.2 % (2 of 91) | 2.2 % (2 of 91) |
1. Galectin-3-LBT
MLIVPYNLPL PGGVVPRMLI TILGTVKPNA NRIALDFQRG NDVAFHFNPR FNERNRRVIV CNTKLDNNWG REERQSVFPF ESGKPFKIQV LVEPDHFKVA VNDAHLLQYN HRVKKLNEIS KLGISGDIDL TSASYTMIYI DTNNDGWYEG DELLASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.4, Details 0.3mM LBT-Galectin-3 in 50mM MES/Nacl buffer,pH=5.4 mixing with 0.3mM Lutetium ion
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Galectin-3-LBT | [U-15N] | 0.3 mM | |
2 | MES | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | Lutetium chloride | natural abundance | 0.3 mM |