BMRBj - BMREntryMaster

Found 1 Document (1.225 seconds)

BMRB: 7428

1F6G

Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR

Authors

Chill, J.H., Louis, J.M., Delaglio, F., Bax, A.

Assembly

KcsA

Entity

1. KcsA (polymer, Thiol state: not present), 145 monomers, 16072.30 × 4 Da Detail

LLGRHGSALH WRAAGAATVL LVIVLLAGSY LAVLAERGAP GAQLITYPRA LWWSVETATT VGYGDLYPVT LWGRLVAVVV MVAGITSFGL VTAALATWFV GREQERRGHF VRHSEKAAEE AYTRTTRALH ERFDRLERML DDNRR

Total weight

64289.2 Da

Max. entity weight

16072.3 Da

Source organism

Streptomyces coelicolor

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 40.3 %, Completeness (bb): 68.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.3 % (664 of 1647)	24.6 % (206 of 836)	48.3 % (319 of 661)	92.7 % (139 of 150)
Backbone	68.6 % (593 of 864)	51.7 % (155 of 300)	70.9 % (299 of 422)	97.9 % (139 of 142)
Sidechain	9.4 % (86 of 915)	9.5 % (51 of 536)	9.4 % (35 of 371)	0.0 % (0 of 8)
Aromatic	6.3 % (10 of 160)	6.3 % (5 of 80)	6.7 % (5 of 75)	0.0 % (0 of 5)
Methyl	7.5 % (16 of 214)	7.5 % (8 of 107)	7.5 % (8 of 107)

1. KcsA

LLGRHGSALH WRAAGAATVL LVIVLLAGSY LAVLAERGAP GAQLITYPRA LWWSVETATT VGYGDLYPVT LWGRLVAVVV MVAGITSFGL VTAALATWFV GREQERRGHF VRHSEKAAEE AYTRTTRALH ERFDRLERML DDNRR

Show sample condition

Sample

Solvent system SDS

#	Name	Isotope labeling	Type	Concentration
1	KcsA	natural abundance		0.5-0.6 mM
2	MES	natural abundance		25 mM

Release date

2008-08-06

Citation 1

Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR
Chill, J.H., Louis, J.M., Delaglio, F., Bax, A.
Biochim. Biophys. Acta (2007), 1768, 3260-3270, PubMed 17945182 , DOI 10.1016/j.bbamem.2007.08.006 , Abstract

Show more 1 citaion

Citation 2

NMR study of the tetrameric KcsA potassium channel in detergent micelles
Chill, J.H., Louis, J.M., Miller, C., Bax, A.
Protein Sci. (2006), 15, 684-698, PubMed 16522799 , DOI 10.1110/ps.051954706 , Abstract

Hide non-primary 1 citaion

Related entities 1. KcsA, : 1 : 1 : 1 : 46 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7428_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7428_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_4	Warning	562		98.6 (chain: 1, length: 145)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_4
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 145, Sequence coverage: 98.6%
- Total number of assignments
  - ¹H chemical shifts: 139
  - ¹³C chemical shifts: 284
  - ¹⁵N chemical shifts: 139
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	836	139	16.6
¹³C chemical shifts	661	284	43.0
¹⁵N chemical shifts	166	139	83.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	300	139	46.3
¹³C chemical shifts	290	284	97.9
¹⁵N chemical shifts	142	139	97.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	536	0	0.0
¹³C chemical shifts	371	0	0.0
¹⁵N chemical shifts	24	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	109	0	0.0
¹³C chemical shifts	109	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	80	0	0.0
¹³C chemical shifts	75	0	0.0
¹⁵N chemical shifts	5	0	0.0

Keywords KcsA, NMR, potassium channel

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Found 1 Document (1.225 seconds)

BMRB: 7428

Sample

Related entities 1. KcsA, : 1 : 1 : 1 : 46 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail