BMRB: 7429

Backbone 1H and 15N Chemical Shift Assignments for the Phosphotyrosine Binding Domain of Insulin Receptor Substrate 1 in the Apo and Phosphopeptide Bound Forms

Authors

Stone, M.J., Jarymowycz, V.A.

Assembly

Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 Monomer complex with Interleukin-4 Receptor Phosphopeptide

Entity

1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1 (polymer, Thiol state: all free), 111 monomers, 12502.42 Da Detail

GPAFKEVWQV ILKPKGLGQT KNLIGIYRLC LTSKTISFVK LNSEAAAVVL QLMNIRRCGH SENFFFIEVG RSAVTGPGEF WMQVDDSVVA QNMHETILEA MRAMSDEFRP R

2. Interleukin-4 Receptor Phosphopeptide (polymer, Thiol state: not present), 11 monomers, 1240.302 Da Detail

LVIAGNPAXR S

Total weight

13742.722 Da

Max. entity weight

12502.42 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 78.7 %, Completeness: 23.3 %, Completeness (bb): 53.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	23.3 % (202 of 868)	14.1 % (104 of 739)	76.0 % (98 of 129)
Backbone	53.6 % (194 of 362)	39.8 % (98 of 246)	82.8 % (96 of 116)
Sidechain	1.6 % (8 of 506)	1.2 % (6 of 493)	15.4 % (2 of 13)
Aromatic	7.0 % (4 of 57)	3.6 % (2 of 55)	100.0 % (2 of 2)
Methyl	0.0 % (0 of 73)	0.0 % (0 of 73)

1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1

GPAFKEVWQV ILKPKGLGQT KNLIGIYRLC LTSKTISFVK LNSEAAAVVL QLMNIRRCGH SENFFFIEVG RSAVTGPGEF WMQVDDSVVA QNMHETILEA MRAMSDEFRP R

2. Interleukin-4 Receptor Phosphopeptide

LVIAGNPAXR S

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details A complex between IRS-PTB and the IL-4 receptor peptide was formed by addition of peptide under the same buffer conditions to the protein to obtain a stoichiometery of 1:1.5 (protein:peptide).

#	Name	Isotope labeling	Type	Concentration
1	Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1	[U-15N]		200 uM

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Release date

2008-10-02

Citation

Remote changes in the dynamics of the phosphotyrosine-binding domain of insulin receptor substrate-1 induced by phosphopeptide binding
Stone, M.J., Jarymowycz, V.A.
Biochemistry (2008), 47, 13371-13382, PubMed 19053277 , DOI 10.1021/bi801096b , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1, : 1 : 8 : 1 : 34 entities Detail

Related entities 2. Interleukin-4 Receptor Phosphopeptide, : 1 entities Detail

Interaction partners 1. Phosphotyrosine-Binding Domain of Insulin Receptor Substrate-1, : 19 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail