BMRB: 7430

Solution structure of Reduced ERp18

Authors

Rowe, M.L., Alanen, H.I., Ruddock, L.W., Kelly, G., Schmidt, J.M., Williamson, R.A., Howard, M.J.

Assembly

ERp18

Entity

1. ERp18 (polymer, Thiol state: all free), 157 monomers, 17770.70 Da Detail

MHHHHHHMSD GHNGLGKGFG DHIHWRTLED GKKEAAASGL PLMVIIHKSW CGACKALKPK FAESTEISEL SHNFVMVNLE DEEEPKDEDF SPDGGYIPRI LFLDPSGKVH PEIINENGNP SYKYFYVSAE QVVQGMKEAQ ERLTGDAFRK KHLEDEL

Formula weight

17770.7 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 36.1 %, Completeness (bb): 63.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	36.1 % (661 of 1830)	21.0 % (201 of 956)	43.0 % (307 of 714)	95.6 % (153 of 160)
Backbone	63.5 % (588 of 926)	46.9 % (150 of 320)	65.0 % (297 of 457)	94.6 % (141 of 149)
Sidechain	19.8 % (207 of 1047)	8.0 % (51 of 636)	36.3 % (145 of 400)	100.0 % (11 of 11)
Aromatic	3.9 % (7 of 178)	4.5 % (4 of 89)	1.1 % (1 of 87)	100.0 % (2 of 2)
Methyl	13.6 % (18 of 132)	6.1 % (4 of 66)	21.2 % (14 of 66)

1. ERp18

MHHHHHHMSD GHNGLGKGFG DHIHWRTLED GKKEAAASGL PLMVIIHKSW CGACKALKPK FAESTEISEL SHNFVMVNLE DEEEPKDEDF SPDGGYIPRI LFLDPSGKVH PEIINENGNP SYKYFYVSAE QVVQGMKEAQ ERLTGDAFRK KHLEDEL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Sample Reduced prior to concentration using 5 mM DTT

#	Name	Isotope labeling	Type	Concentration
1	ERp18	[U-99% 15N]		0.7-1.5 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 %
4	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details Sample Reduced prior to concentration using 5 mM DTT

#	Name	Isotope labeling	Type	Concentration
5	ERp18	[U-99% 13C; U-99% 15N]		0.7-1.5 mM
6	sodium phosphate	natural abundance		20 mM
7	sodium chloride	natural abundance		100 %
8	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 40 models in PDB: 2K8V, Strand ID: A Detail

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Release date

2009-04-21

Citation 1

Solution structure and dynamics of ERp18, a small endoplasmic reticulum resident oxidoreductase
Rowe, M.L., Ruddock, L.W., Kelly, G., Schmidt, J.M., Williamson, R.A., Howard, M.J.
Biochemistry (2009), 48, 4596-4606, PubMed 19361226 , DOI 10.1021/bi9003342 , Abstract

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Citation 2

Functional characterization of ERp18, a new endoplasmic reticulum-located thioredoxin superfamily member
Alanen, H.I., Williamson, R.A., Howard, M.J., Lappi, A., Jantti, H.P., Rautio, S.M., Kellokupu, S., Ruddock, L.W.
J. Biol. Chem. (2003), 278, 28912-28920, PubMed 12761212 , DOI 10.1074/jbc.M304598200 , Abstract

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Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. ERp18, : 1 : 2 : 34 entities Detail

Interaction partners 1. ERp18, : 6 interactors Detail

Experiments performed 3 experiments Detail