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Assignment of the Backbone 1H and 15N NMR Resonances of Bacteriophage T4 Lysozyme
Authors
McIntosh, L.P., Wand, A.JOSHUA., Lowry, D.F., Redfield, A.G., Dahlquist, F.W.
Assembly
T4 lysozyme
Entity
1. T4 lysozyme (polymer), 164 monomers, 18635.23 Da Detail

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNCNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRCALI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL


Formula weight
18635.23 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.2 %, Completeness: 69.3 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

Total1H15N
All69.3 % (837 of 1207)66.1 % (670 of 1013)86.1 % (167 of 194)
Backbone98.6 % (479 of 486)97.8 % (318 of 325)100.0 % (161 of 161)
Sidechain49.7 % (358 of 721)51.2 % (352 of 688)18.2 % (6 of 33)
Aromatic94.4 % (68 of 72)94.2 % (65 of 69)100.0 % (3 of 3)
Methyl50.0 % (48 of 96)50.0 % (48 of 96)

1. T4 lysozyme

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNCNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRCALI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Sample

Temperature 293 K, pH 5.6



Release date
1995-07-30
Citation
Assignment of the backbone 1H and 15N NMR resonances of bacteriophage T4 lysozyme
McIntosh, L.P., Wand, A.JOSHUA., Lowry, D.F., Redfield, A.G., Dahlquist, F.W.
Biochemistry (1990), 29, 6341-6362, PubMed 2207079 , DOI 10.1021/bi00479a003 ,
Related entities 1. T4 lysozyme, : 1 : 7 : 19 : 74 : 9 entities Detail
Experiments performed 1 experiments Detail