BMRBj - BMREntryMaster

Found 1 Document (0.571 seconds)

BMRB: 915

4LZM

Assignment of the Backbone 1H and 15N NMR Resonances of Bacteriophage T4 Lysozyme

Authors

McIntosh, L.P., Wand, A.JOSHUA., Lowry, D.F., Redfield, A.G., Dahlquist, F.W.

Assembly

T4 lysozyme

Entity

1. T4 lysozyme (polymer), 164 monomers, 18635.23 Da Detail

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNCNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRCALI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Formula weight

18635.23 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 68.4 %, Completeness (bb): 98.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	68.4 % (817 of 1194)	64.5 % (653 of 1013)	90.6 % (164 of 181)
Backbone	98.2 % (488 of 497)	97.3 % (327 of 336)	100.0 % (161 of 161)
Sidechain	47.2 % (329 of 697)	48.2 % (326 of 677)	15.0 % (3 of 20)
Aromatic	94.4 % (68 of 72)	94.2 % (65 of 69)	100.0 % (3 of 3)
Methyl	47.9 % (46 of 96)	47.9 % (46 of 96)

1. T4 lysozyme

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNCNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRCALI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Show sample condition

Sample

Temperature 293 K, pH 5.6

Release date

1995-07-30

Citation

Assignment of the backbone 1H and 15N NMR resonances of bacteriophage T4 lysozyme
McIntosh, L.P., Wand, A.JOSHUA., Lowry, D.F., Redfield, A.G., Dahlquist, F.W.
Biochemistry (1990), 29, 6341-6362, PubMed 2207079 , DOI: , Abstract

Related entities 1. T4 lysozyme, : 1 : 7 : 19 : 77 : 9 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr915_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr915_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	885		98.2 (chain: 1, length: 164)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 164, Sequence coverage: 98.2%
- Total number of assignments
  - ¹⁵N chemical shifts: 166
  - ¹H chemical shifts: 719
- Completeness of assignments
  - Entity_assemble_ID: 1
- Tautomeric state of HIS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
31	HIS	ND1	188.0
31	HIS	NE2	174.4
152	THR	HG1	5.54
161	TYR	HH	11.29

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	194	164	84.5
¹H chemical shifts	1013	633	62.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	33	3	9.1
¹H chemical shifts	677	306	45.2

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Found 1 Document (0.571 seconds)

BMRB: 915

Sample

Related entities 1. T4 lysozyme, : 1 : 7 : 19 : 77 : 9 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	161	161	100.0
¹H chemical shifts	336	327	97.3