BMRBj - BMREntryMaster

Found 1 Document (0.633 seconds)

BMRB: 922

2HV4

Assignment of Proton Resonances, Identification of Secondary Structural Elements, and Analysis of Backbone Chemical Shifts for the C102T Variant of Yeast Iso-1-cytochrome c and Horse Cytochrome c

Authors

Gao, Y., Boyd, J., Williams, R.J.P., Pielak, G.J.

Assembly

cytochrome c

Entity

1. cytochrome c (polymer), 108 monomers, 12091.69 Da Detail

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPXKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

2. HEME C (non-polymer), 618.503 Da

Total weight

12710.193 Da

Max. entity weight

12091.69 Da

Entity Connection

covalent 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:CYS19:SG	2:HEC1:na
2	covalent	sing	1:CYS22:SG	2:HEC1:na

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 79.1 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	79.1 % (523 of 661)	79.1 % (523 of 661)
Backbone	95.0 % (211 of 222)	95.0 % (211 of 222)
Sidechain	71.1 % (312 of 439)	71.1 % (312 of 439)
Aromatic	85.2 % (46 of 54)	85.2 % (46 of 54)
Methyl	95.7 % (44 of 46)	95.7 % (44 of 46)

1. cytochrome c

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPXKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample

Temperature 300 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	na

Release date

2015-08-05

Citation

Assignment of proton resonances, identification of secondary structural elements, and analysis of backbone chemical shifts for the C102T variant of yeast iso-1-cytochrome c and horse cytochrome c
Gao, Y., Boyd, J., Williams, R.J.P., Pielak, G.J.
Biochemistry (1990), 29, 6994-7003, PubMed 2171638 , DOI 10.1021/bi00482a007 , Abstract

Related entities 1. cytochrome c, : 1 : 1 : 7 : 66 : 161 entities Detail

Interaction partners 1. cytochrome c, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr922_3.str
Diamagnetism of the molecular assembly	False (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr922_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	610		94.4 (chain: 1, length: 108)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 108, Sequence coverage: 94.4%
- Total number of assignments
  - ¹H chemical shifts: 610
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
43	ARG	HH11	9.12
43	ARG	HH12	9.12
43	ARG	HH21	9.12
43	ARG	HH22	9.12
51	TYR	HH	9.31
53	TYR	HH	9.78

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Found 1 Document (0.633 seconds)

BMRB: 922

Sample

Related entities 1. cytochrome c, : 1 : 1 : 7 : 66 : 161 entities Detail

Interaction partners 1. cytochrome c, : 4 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 4 contents Detail