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Assignment of Proton Resonances, Identification of Secondary Structural Elements, and Analysis of Backbone Chemical Shifts for the C102T Variant of Yeast Iso-1-cytochrome c and Horse Cytochrome c
Authors
Gao, Y., Boyd, J., Williams, R.J.P., Pielak, G.J.
Assembly
cytochrome c
Entity
1. cytochrome c (polymer), 108 monomers, 12091.69 Da Detail

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPXKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE


2. HEME C (non-polymer), 618.503 Da
Total weight
12710.193 Da
Max. entity weight
12091.69 Da
Entity Connection
covalent 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1covalentsing1:CYS19:SG2:HEC1:na
2covalentsing1:CYS22:SG2:HEC1:na

Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.4 %, Completeness: 76.6 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

Total1H
All76.6 % (506 of 661)76.6 % (506 of 661)
Backbone95.7 % (201 of 210)95.7 % (201 of 210)
Sidechain67.6 % (305 of 451)67.6 % (305 of 451)
Aromatic85.2 % (46 of 54)85.2 % (46 of 54)
Methyl95.7 % (44 of 46)95.7 % (44 of 46)

1. cytochrome c

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPXKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Sample

Temperature 300 K, pH 7.2


#NameIsotope labelingTypeConcentration
1cytochrome c

Release date
2015-06-01
Citation
Assignment of proton resonances, identification of secondary structural elements, and analysis of backbone chemical shifts for the C102T variant of yeast iso-1-cytochrome c and horse cytochrome c
Gao, Y., Boyd, J., Williams, R.J.P., Pielak, G.J.
Biochemistry (1990), 29, 6994-7003, PubMed 2171638 , DOI 10.1021/bi00482a007 ,
Related entities 1. cytochrome c, : 1 : 1 : 7 : 52 : 139 entities Detail
Interaction partners 1. cytochrome c, : 4 interactors Detail
Experiments performed 1 experiments Detail