BMRB: 960

High Resolution Nuclear Magnetic Resonance Studies of the Active Site of Chymotrypsin II. Polarization of Histidine 57 by Substrate Analogues and Competitive Inhibitors

Authors

Robillard, G., Shulman, R.G.

Assembly

chymotrypsinogen A

Entity

1. chymotrypsinogen A (polymer), 245 monomers, 25663.86 Da Detail

CGVPAIQPVL SGLSRIVNGE EAVPGSWPWQ VSLQDKTGFH FCGGSLINEN WVVTAAHCGV TTSDVVVAGE FDQGSSSEKI QKLKIAKVFK NSKYNSLTIN NDITLLKLST AASFSQTVSA VCLPSASDDF AAGTTCVVTG WGLTRYTNAN TPDRLQQASL PLLSNTNCKK YWGTKIKDAM ICAGASGVSS CMGDSGGPLV CKKNGAWTLV GIVSWGSSTC STSTPGVYAR VTALVNWVQQ TLAAN

Formula weight

25663.86 Da

Source organism

Bos primigenius

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 3.3 %, Completeness: 1.8 %, Completeness (bb): 1.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	1.8 % (24 of 1359)	1.8 % (24 of 1359)
Backbone	1.8 % (9 of 504)	1.8 % (9 of 504)
Sidechain	1.8 % (15 of 855)	1.8 % (15 of 855)
Aromatic	0.0 % (0 of 98)	0.0 % (0 of 98)
Methyl	0.0 % (0 of 150)	0.0 % (0 of 150)

1. chymotrypsinogen A

CGVPAIQPVL SGLSRIVNGE EAVPGSWPWQ VSLQDKTGFH FCGGSLINEN WVVTAAHCGV TTSDVVVAGE FDQGSSSEKI QKLKIAKVFK NSKYNSLTIN NDITLLKLST AASFSQTVSA VCLPSASDDF AAGTTCVVTG WGLTRYTNAN TPDRLQQASL PLLSNTNCKK YWGTKIKDAM ICAGASGVSS CMGDSGGPLV CKKNGAWTLV GIVSWGSSTC STSTPGVYAR VTALVNWVQQ TLAAN

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Sample

Temperature 277 K, pH 9.5

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Release date

1995-07-30

Citation

High resolution nuclear magnetic resonance studies of the active site of chymotrypsin. II. Polarization of histidine 57 by substrate analogues and competitive inhibitors
Robillard, G., Shulman, R.G.
J. Mol. Biol. (1974), 86, 541-558, PubMed 4852270 , Abstract

Related entities 1. chymotrypsinogen A, : 1 : 32 : 212 entities Detail

Interaction partners 1. chymotrypsinogen A, : 6 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail