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High Resolution Nuclear Magnetic Resonance Studies of the Active Site of Chymotrypsin II. Polarization of Histidine 57 by Substrate Analogues and Competitive Inhibitors
Authors
Robillard, G., Shulman, R.G.
Assembly
chymotrypsinogen A
Entity
1. chymotrypsinogen A (polymer), 245 monomers, 25663.86 Da Detail

CGVPAIQPVL SGLSRIVNGE EAVPGSWPWQ VSLQDKTGFH FCGGSLINEN WVVTAAHCGV TTSDVVVAGE FDQGSSSEKI QKLKIAKVFK NSKYNSLTIN NDITLLKLST AASFSQTVSA VCLPSASDDF AAGTTCVVTG WGLTRYTNAN TPDRLQQASL PLLSNTNCKK YWGTKIKDAM ICAGASGVSS CMGDSGGPLV CKKNGAWTLV GIVSWGSSTC STSTPGVYAR VTALVNWVQQ TLAAN


Formula weight
25663.86 Da
Source organism
Bos taurus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 2.4 %, Completeness: 1.4 %, Completeness (bb): 1.9 % Detail

Polymer type: polypeptide(L)

Total1H
All 1.4 % (19 of 1359) 1.4 % (19 of 1359)
Backbone 1.9 % (9 of 481) 1.9 % (9 of 481)
Sidechain 1.1 % (10 of 878) 1.1 % (10 of 878)
Aromatic 0.0 % (0 of 98) 0.0 % (0 of 98)
Methyl 0.7 % (1 of 150) 0.7 % (1 of 150)

1. chymotrypsinogen A

CGVPAIQPVL SGLSRIVNGE EAVPGSWPWQ VSLQDKTGFH FCGGSLINEN WVVTAAHCGV TTSDVVVAGE FDQGSSSEKI QKLKIAKVFK NSKYNSLTIN NDITLLKLST AASFSQTVSA VCLPSASDDF AAGTTCVVTG WGLTRYTNAN TPDRLQQASL PLLSNTNCKK YWGTKIKDAM ICAGASGVSS CMGDSGGPLV CKKNGAWTLV GIVSWGSSTC STSTPGVYAR VTALVNWVQQ TLAAN

Sample

Temperature 277 K, pH 9.5



Release date
1995-07-30
Citation
High resolution nuclear magnetic resonance studies of the active site of chymotrypsin. II. Polarization of histidine 57 by substrate analogues and competitive inhibitors
Robillard, G., Shulman, R.G.
J. Mol. Biol. (1974), 86, 541-558, PubMed 4852270 , DOI 10.1016/0022-2836(74)90179-x ,
Related entities 1. chymotrypsinogen A, : 1 : 26 : 105 entities Detail
Interaction partners 1. chymotrypsinogen A, : 6 interactors Detail
Experiments performed 1 experiments Detail