BMRB: 967

High Resolution Nuclear Magnetic Resonance Studies of the Active Site of Chymotrypsin II. Polarization of Histidine 57 by Substrate Analogues and Competitive Inhibitors

Authors

Robillard, G., Shulman, R.G.

Assembly

chymotrypsin

Entity

1. chymotrypsin (polymer), 230 monomers, 24185.10 Da Detail

IVNGEEAVPG SWPWQVSLQD KTGFHFCGGS LINENWVVTA AHCGVTTSDV VVAGEFDQGS SSEKIQKLKI AKVFKNSKYN SLTINNDITL LKLSTAASFS QTVSAVCLPS ASDDFAAGTT CVVTGWGLTR YTNANTPDRL QQASLPLLSN TNCKKYWGTK IKDAMICAGA SGVSSCMGDS GGPLVCKKNG AWTLVGIVSW GSSTCSTSTP GVYARVTALV NWVQQTLAAN

Formula weight

24185.1 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 0.9 %, Completeness: 0.9 %, Completeness (bb): 0.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	0.9 % (11 of 1276)	0.9 % (11 of 1276)
Backbone	0.8 % (4 of 474)	0.8 % (4 of 474)
Sidechain	0.9 % (7 of 802)	0.9 % (7 of 802)
Aromatic	0.0 % (0 of 98)	0.0 % (0 of 98)
Methyl	0.0 % (0 of 139)	0.0 % (0 of 139)

1. chymotrypsin

IVNGEEAVPG SWPWQVSLQD KTGFHFCGGS LINENWVVTA AHCGVTTSDV VVAGEFDQGS SSEKIQKLKI AKVFKNSKYN SLTINNDITL LKLSTAASFS QTVSAVCLPS ASDDFAAGTT CVVTGWGLTR YTNANTPDRL QQASLPLLSN TNCKKYWGTK IKDAMICAGA SGVSSCMGDS GGPLVCKKNG AWTLVGIVSW GSSTCSTSTP GVYARVTALV NWVQQTLAAN

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Sample

Temperature 277 K, pH 9.5

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Release date

1995-07-30

Citation

High resolution nuclear magnetic resonance studies of the active site of chymotrypsin. II. Polarization of histidine 57 by substrate analogues and competitive inhibitors
Robillard, G., Shulman, R.G.
J. Mol. Biol. (1974), 86, 541-558, PubMed 4852270 , Abstract

Related entities 1. chymotrypsin, : 1 : 32 : 210 entities Detail

Interaction partners 1. chymotrypsin, : 6 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail