BMRB: 21008

LPS bound structure of Temporin-1Tl

Authors

Bhattacharyya, S.

Assembly

TemporinL

Entity

1. TemporinL (polymer, Thiol state: not present), 13 monomers, 1640.966 × 2 Da Detail

FVQWFSKFLG RIL

Total weight

3281.932 Da

Max. entity weight

1640.966 Da

Source organism

Rana temporaria

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, conformer_family_coord_set

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.5 %, Completeness (bb): 96.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	84.5 % (82 of 97)	84.5 % (82 of 97)
Backbone	96.3 % (26 of 27)	96.3 % (26 of 27)
Sidechain	80.0 % (56 of 70)	80.0 % (56 of 70)
Aromatic	66.7 % (14 of 21)	66.7 % (14 of 21)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. entity

FVQWFSKFLG RIL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 290 K, pH 4.5, Details 0.5mM TemporinL and 14.5micro molar of E.coli LPS

#	Name	Isotope labeling	Type	Concentration
1	TemporinL	natural abundance		0.5 mM
2	LPS	natural abundance		14.5 mM
3	H20	natural abundance		90 %
4	D20	natural abundance		10 %

Hide sample codition

Release date

2011-07-10

Citation

NMR structures and interactions of temporin-1Tl and temporin-1Tb with lipopolysaccharide micelles: mechanistic insights into outer membrane permeabilization and synergistic activity
Bhunia, A., Saravanan, R., Mohanram, H., Mangoni, M.L., Bhattacharjya, S.
J. Biol. Chem. (2011), 286, 24394-24406, PubMed 21586570 , DOI 10.1074/jbc.M110.189662 , Abstract

Related entities 1. TemporinL, : 1 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail