1H and 15N assignment of oxidized Pseudomonas aeruginosa cytochrome c551
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | thioether | sing | 1:CYS12:SG | 2:HEC1:CAB |
2 | thioether | sing | 1:CYS15:SG | 2:HEC1:CAC |
3 | metal coordination | sing | 1:HIS16:NE | 2:HEC1:FE |
4 | metal coordination | sing | 1:MET61:SD | 2:HEC1:FE |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 28.4 % (160 of 563) | 18.0 % (86 of 477) | 86.0 % (74 of 86) |
Backbone | 63.1 % (152 of 241) | 47.3 % (78 of 165) | 97.4 % (74 of 76) |
Sidechain | 2.5 % (8 of 322) | 2.6 % (8 of 312) | 0.0 % (0 of 10) |
Aromatic | 0.0 % (0 of 30) | 0.0 % (0 of 28) | 0.0 % (0 of 2) |
Methyl | 0.0 % (0 of 43) | 0.0 % (0 of 43) |
1. cytochrome c551
EDPEVLFKNK GCVACHAIDT KMVGPAYKDV AAKFAGQAGA EAELAQRIKN GSQGVWGPIP MPPNAVSDDE AQTLAKWVLS QKTemperature 296 (±2) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c551 | [U-15N] | 1 mM | |
2 | potassium phosphate | 20 mM | ||
3 | potassium ferricyanide | 10 mM |