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Found 1 Document (0.971 seconds)

BMRB: 11362

2E61

Assigned chemical shifts of the zf-CW domain in zinc finger CW-type PWWP domain protein 1

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Zinc finger CW-type PWWP domain protein 1

Entity

1. zf-CW domain (polymer, Thiol state: free and other bound), 69 monomers, 7644.315 Da Detail

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE

2. ZN (non-polymer), 65.409 Da

Total weight

7709.724 Da

Max. entity weight

7644.315 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS21:SG	2:ZN1:ZN
2	na	sing	1:CYS26:SG	2:ZN1:ZN
3	na	sing	1:CYS47:SG	2:ZN1:ZN
4	na	sing	1:CYS58:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.8 %, Completeness: 94.0 %, Completeness (bb): 90.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.0 % (720 of 766)	95.2 % (379 of 398)	94.1 % (273 of 290)	87.2 % (68 of 78)
Backbone	90.1 % (366 of 406)	90.8 % (129 of 142)	91.5 % (182 of 199)	84.6 % (55 of 65)
Sidechain	97.9 % (412 of 421)	97.7 % (250 of 256)	98.0 % (149 of 152)	100.0 % (13 of 13)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (34 of 34)	100.0 % (4 of 4)
Methyl	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (25 of 25)

1. zf-CW domain

GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2+1mM {IDA;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	zf-CW domain		protein	0.0 ~ 0.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	50 uM
7	IDA	natural abundance	salt	1 mM
8	H2O		solvent	90 %
9	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2E61, Strand ID: A Detail

Release date

2011-01-03

Citation

Structural insight into the zinc finger CW domain as a histone modification reader
He, F., Umehara, T., Saito, K., Harada, T., Watanabe, S., Yabuki, T., Kigawa, T., Takahashi, M., Kuwasako, K., Tsuda, K., Matsuda, T., Aoki, M., Seki, E., Kobayashi, N., Guntert, P., Yokoyama, S., Muto, Y.
Structure (2010), 18, 1127-1139, PubMed 20826339 , DOI 10.1016/j.str.2010.06.012 , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 11358 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H3 peptide
  BMRB: 11359 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H3 dimethyl K4 peptide
  BMRB: 11360 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H3 trimethyl K4 peptide
  BMRB: 11361 released on 2011-01-03
    Title Assigned chemical shifts of the zf-CW domain with H4 trimethyl K20 peptide
  BMRB: 11115 released on 2010-09-30
    Title Complex structure of the zf-CW domain and the H3K4me3 peptide

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Found 1 Document (0.971 seconds)

BMRB: 11362

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. zf-CW domain, : 1 : 1 : 2 : 10 entities Detail

Interaction partners 1. zf-CW domain, : 3 interactors Detail

Experiments performed 2 experiments Detail

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Instrument

Sample