BMRBj - BMREntryMaster

Found 1 Document (1.138 seconds)

BMRB: 15048

2L0X

Rub184

Authors

Berghaus, C., Schwarten, M., Heumann, R., Stoll, R.

Assembly

Rub184GDP

Entity

1. Rub184 (polymer, Thiol state: all free), 184 monomers, 20435.08 Da Detail

SPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF RRIILEAEKI DGAASQGKSS CSVM

2. GDP (non-polymer), 443.201 Da

Total weight

20878.281 Da

Max. entity weight

20435.08 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 69.3 %, Completeness (bb): 90.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.3 % (1492 of 2152)	68.7 % (764 of 1112)	66.3 % (559 of 843)	85.8 % (169 of 197)
Backbone	90.2 % (989 of 1096)	90.4 % (340 of 376)	90.0 % (486 of 540)	90.6 % (163 of 180)
Sidechain	53.4 % (656 of 1228)	57.7 % (425 of 736)	47.4 % (225 of 475)	35.3 % (6 of 17)
Aromatic	10.8 % (17 of 158)	20.3 % (16 of 79)	0.0 % (0 of 78)	100.0 % (1 of 1)
Methyl	67.5 % (154 of 228)	78.1 % (89 of 114)	57.0 % (65 of 114)

1. Rub184

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 8

#	Name	Isotope labeling	Concentration
1	GTP-binding protein	[U-15N]	0.9 ~ 1.2 mM
2	D2O		10 %
3	potassium phosphate		20 mM
4	H2O		90 %
5	DTT		1 mM
6	GUANOSINE-5'-DIPHOSPHATE		0.9 ~ 1.2 mM
7	sodium chloride		10 mM
8	TSP		1 mM
9	magnesium chloride		5 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 8

#	Name	Isotope labeling	Concentration
10	GTP-binding protein	[U-13C; U-15N]	0.9 ~ 1.2 mM
11	GUANOSINE-5'-DIPHOSPHATE		0.9 ~ 1.2 mM
12	D2O		10 %
13	H2O		90 %
14	DTT		1 mM
15	sodium chloride		10 mM
16	potassium phosphate		20 mM
17	TSP		1 mM
18	magnesium chloride		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	1.0
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 1 Detail

Release date

2007-11-20

Citation 1

Sequence-specific 1H, 13C, and 15N backbone assignment of the GTPase rRheb in its GDP-bound form
Berghaus, C., Schwarten, M., Heumann, R., Stoll, R.
Biomol. NMR Assign. (2007), 1, 45-47, PubMed 19636822 , DOI 10.1007/s12104-007-9013-4 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. Rub184, : 1 : 3 : 274 entities Detail

Interaction partners 1. Rub184, : 22 interactors Detail

More details for 22 interactors identified by 13 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC