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Backbone and side chain chemical shift assignments of the F153-to-5-flurotryptophan mutant of human cardiac troponin C
Authors
Wang, X., Mercier, P., Letourneau, P., Sykes, B.D.
Assembly
F153(FTR) cTnC
Entity
1. F153(FTR) (polymer, Thiol state: not present), 161 monomers, 18427.23 Da Detail

MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEXLEFMKGV E


2. CA (non-polymer), 40.078 Da
Total weight
18467.309 Da
Max. entity weight
18427.23 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.5 %, Completeness: 75.9 %, Completeness (bb): 81.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All75.9 % (1398 of 1842)76.8 % (737 of 960)71.7 % (509 of 710)88.4 % (152 of 172)
Backbone81.7 % (771 of 944)95.6 % (304 of 318)67.5 % (316 of 468)95.6 % (151 of 158)
Sidechain73.8 % (772 of 1046)67.6 % (434 of 642)86.4 % (337 of 390) 7.1 % (1 of 14)
Aromatic24.0 % (25 of 104)25.0 % (13 of 52)23.1 % (12 of 52)
Methyl100.0 % (148 of 148)100.0 % (74 of 74)100.0 % (74 of 74)

1. F153(FTR)

MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEXLEFMKGV E

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.5) K, pH 6.7 (±0.2)


#NameIsotope labelingTypeConcentration
1F153(FTR)natural abundance1.0 (±0.2) mM
2Calciumnatural abundance10 (±1.0) mM
3potassium chloridenatural abundance100 (±10.0) mM
4H2O90 mM
5D2O10 mM

LACS Plot; CA
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2JT8, Strand ID: A Detail


Release date
2007-08-12
Citation
Effects of Phe-to-Trp mutation and fluorotryptophan incorporation on the solution structure of cardiac troponin C, and analysis of its suitability as a potential probe for in situ NMR studies
Wang, X., Mercier, P., Letourneau, P., Sykes, B.
Protein Sci. (2005), 14, 2447-2460, PubMed 16131667 , DOI 10.1110/ps.051595805 ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 15427 released on 2008-03-13
    Title Chemical shift assignments of the F104-to-5-flurotryptophan mutant of cardiac troponin C
  BMRB: 15388 released on 2008-03-12
    Title Solution Structure of F153W cardiac troponin C
  BMRB: 15385 released on 2007-08-14
    Title Solution structure of F104W cardiac troponin C
Related entities 1. F153(FTR), : 1 : 2 : 67 entities Detail
Experiments performed 6 experiments Detail
Keywords calcium-binding protein, EF-hand protein, muscle regulatory protein, Trp mutation, Calcium-bind protein, Phe-to-Trp mutation