BMRB: 15904

1J coupling constants related to the Ca carbons in oxidised Flavodoxin from Desulfovibrio vulgaris (Hildenborough)

Authors

Schmidt, J.M., Lohr, F.

Assembly

Flavodoxin oxidized

Entity

1. Flavodoxin (polymer, Thiol state: all free), 147 monomers, 15692.04 Da Detail

PKALIVYGST TGNTEYTAET IARELADAGY EVDSRDAASV EAGGLFEGFD LVLLGCSTWG DDSIELQDDF IPLFDSLEET GAQGRKVACF GCGDSSYEYF CGAVDAIEEK LKNLGAEIVQ DGLRIDGDPR AARDDIVGWA HDVRGAI

2. FMN (non-polymer), 456.344 Da

Total weight

16148.384 Da

Max. entity weight

15692.04 Da

Source organism

Desulfovibrio vulgaris

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

535 J values in 4 lists
Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 300 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 300 K, pH 7.0

Experiment name 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA, 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 3D Ca-coupled HA(CACO)NH, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 3D Ha-coupled ct-[15N,1H]-TROSY-HNCA, 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA, 3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation, 3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA

List #1 1JCaCO, Field strength (¹H) 500 MHz

List #2 1JCaN_, Field strength (¹H) 800 MHz

List #3 1JCaHa, Field strength (¹H) 600 MHz, Details 28-ms constant-time Ca evolution in 3D TROSY experiments

List #4 1JCaCb, Field strength (¹H) 800 MHz, Details Ca evolution time typically 70-90ms

Release date

2008-11-18

Citation 1

Variation in protein C(alpha)-related one-bond J couplings
Schmidt, J.M., Howard, M.J., Maestre-Martinez, M., Perez, C.S., Lohr, F.
Magn. Reson. Chem. (2009), 47, 16-30, PubMed 18853398 , DOI 10.1002/mrc.2337 , Abstract

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Citation 2

Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N
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Citation 3

TROSY in triple-resonance experiments: new perspectives for sequential NMR assignment of large proteins
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Proc. Natl. Acad. Sci. U. S. A. (1998), 95, 13585-13590, PubMed 9811843 , Abstract

Citation 4

Recording heteronuclear quantitative J-correlation spectra with internal reference peaks
Lohr, F., Perez, C., Schmidt, J.M., Ruterjans, H.
Bull. Magn. Reson. (1999), 20, 9-14

Citation 5

TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins
Salzmann, M., Wider, G., Pervushin, K., Senn, H., Wuthrich, K.
J. Am. Chem. Soc. (1999), 121, 844-848, DOI 10.1021/ja9834226

Citation 6

TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins
Yang, D., Venters, R.A., Mueller, G.A., Choy, W.Y., Kay, L.E.
J. Biomol. NMR (1999), 14, 333-343

Citation 7

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 8

Measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in (15)N,(13)C,(2)H-labeled proteins: application to domain orientation in maltose binding protein
Evenas, J., Mittermaier, A., Yang, D., Kay, L.E.
J. Am. Chem. Soc. (2001), 123, 2858-2864, PubMed 11456973 , DOI: , Abstract

Citation 9

Intraresidue HNCA and COHNCA experiments for protein backbone resonance assignment
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J. Magn. Reson. (2002), 156, 155-159, PubMed 12081454 , Abstract

Citation 10

A novel approach for the sequential backbone assignment of larger proteins: selective intra-HNCA and DQ-HNCA
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Citation 11

Intraresidual HNCA: an experiment for correlating only intraresidual backbone resonances
Permi, P.
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Citation 12

A high sensitivity 3D experiment for measuring Calpha-Halpha residual dipolar coupling constants
Hu, W., Zhang, Z., Chen, Y.
J. Magn. Reson. (2003), 165, 248-252, PubMed 14643706 , DOI 10.1016/j.jmr.2003.08.008 , Abstract

Citation 13

Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract

Citation 14

Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract

Citation 15

Measurement of one-bond 13Calpha-1Halpha residual dipolar coupling constants in proteins by selective manipulation of CalphaHalpha spins
Ball, G., Meenan, N., Bromek, K., Smith, B.O., Bella, J., Uhrin, D.
J. Magn. Reson. (2006), 180, 127-136, PubMed 16495100 , DOI 10.1016/j.jmr.2006.01.017 , Abstract

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Entries sharing articles BMRB: 13 entries Detail

Related entities 1. Flavodoxin, : 1 : 1 : 4 : 19 : 176 entities Detail

Experiments performed 9 experiments Detail