BMRB: 15905

1J coupling constants related to the Ca carbons in Ribonuclease T1 from Aspergillus oryzae

Authors

Schmidt, J.M., Lohr, F.

Assembly

Ribonuclease T1

Entity

1. Ribonuclease T1 (polymer, Thiol state: all disulfide bound), 104 monomers, 11088.64 Da Detail

ACDYTCGSNC YSSSDVSTAQ AAGYKLHEDG ETVGSNSYPH KYNNYEGFDF SVSSPYYEWP ILSSGDVYSG GSPGADRVVF NENNQLAGVI THTGASGNNF VECT

Formula weight

11088.64 Da

Source organism

Aspergillus oryzae

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

360 J values in 4 lists
Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 308 K, pH 5.5 Detail

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Pressure 1 atm, Temperature 308 K, pH 5.5

Experiment name 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 3D Ca-coupled HA(CACO)NH, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA, 3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation, 3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA

List #1 1JCaCO, Field strength (¹H) 500 MHz

List #2 1JCaN_, Field strength (¹H) 600 MHz

List #3 1JCaHa, Field strength (¹H) 600 MHz, Details 28-ms constant-time Ca evolution in 3D TROSY experiments

List #4 1JCaCb, Field strength (¹H) 800 MHz, Details Ca evolution time typically 70-90ms same 3D on all samples 500 or 800

Release date

2008-11-18

Citation 1

Variation in protein C(alpha)-related one-bond J couplings
Schmidt, J.M., Howard, M.J., Maestre-Martinez, M., Perez, C.S., Lohr, F.
Magn. Reson. Chem. (2009), 47, 16-30, PubMed 18853398 , DOI 10.1002/mrc.2337 , Abstract

Show more 12 citaions

Citation 2

Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N
Wang, A.C., Grzesiek, S., Tschudin, R., Lodi, P.J., Bax, A.
J. Biomol. NMR (1995), 5, 376-382, PubMed 7647557 , DOI: , Abstract

Citation 3

TROSY in triple-resonance experiments: new perspectives for sequential NMR assignment of large proteins
Salzmann, M., Pervushin, K., Wider, G., Senn, H., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (1998), 95, 13585-13590, PubMed 9811843 , Abstract

Citation 4

Recording heteronuclear quantitative J-correlation spectra with internal reference peaks
Lohr, F., Perez, C., Schmidt, J.M., Ruterjans, H.
Bull. Magn. Reson. (1999), 20, 9-14

Citation 5

TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins
Salzmann, M., Wider, G., Pervushin, K., Senn, H., Wuthrich, K.
J. Am. Chem. Soc. (1999), 121, 844-848, DOI 10.1021/ja9834226

Citation 6

TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins
Yang, D., Venters, R.A., Mueller, G.A., Choy, W.Y., Kay, L.E.
J. Biomol. NMR (1999), 14, 333-343

Citation 7

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 8

Measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in (15)N,(13)C,(2)H-labeled proteins: application to domain orientation in maltose binding protein
Evenas, J., Mittermaier, A., Yang, D., Kay, L.E.
J. Am. Chem. Soc. (2001), 123, 2858-2864, PubMed 11456973 , DOI: , Abstract

Citation 9

Intraresidue HNCA and COHNCA experiments for protein backbone resonance assignment
Brutscher, B.
J. Magn. Reson. (2002), 156, 155-159, PubMed 12081454 , Abstract

Citation 10

A novel approach for the sequential backbone assignment of larger proteins: selective intra-HNCA and DQ-HNCA
Nietlispach, D., Ito, Y., Laue, E.D.
J. Am. Chem. Soc. (2002), 124, 11199-11207, PubMed 12224968 , DOI: , Abstract

Citation 11

Intraresidual HNCA: an experiment for correlating only intraresidual backbone resonances
Permi, P.
J. Biomol. NMR (2002), 23, 201-209, PubMed 12238592 , Abstract

Citation 12

A high sensitivity 3D experiment for measuring Calpha-Halpha residual dipolar coupling constants
Hu, W., Zhang, Z., Chen, Y.
J. Magn. Reson. (2003), 165, 248-252, PubMed 14643706 , DOI 10.1016/j.jmr.2003.08.008 , Abstract

Citation 13

Measurement of one-bond 13Calpha-1Halpha residual dipolar coupling constants in proteins by selective manipulation of CalphaHalpha spins
Ball, G., Meenan, N., Bromek, K., Smith, B.O., Bella, J., Uhrin, D.
J. Magn. Reson. (2006), 180, 127-136, PubMed 16495100 , DOI 10.1016/j.jmr.2006.01.017 , Abstract

Hide non-primary 12 citaions

Related entities 1. Ribonuclease T1, : 1 : 25 : 11 : 27 : 34 entities Detail

Experiments performed 7 experiments Detail