BMRB: 15909

1J coupling constants related to the Ca carbons in DFPase from Loligo vulgaris

Authors

Schmidt, J.M., Lohr, F.

Assembly

Diisopropylfluorophosphatase

Entity

1. DFPase (polymer, Thiol state: all free), 316 monomers, 35223.49 Da Detail

GSMEIPVIEP LFTKVTEDIP GAEGPVFDKN GDFYIVAPEV EVNGKPAGEI LRIDLKTGKK TVICKPEVNG YGGIPAGCQC DRDANQLFVA DMRLGLLVVQ TDGTFEEIAK KDSEGRRMQG CNDCAFDYEG NLWITAPAGE VAPADYTRSM QEKFGSIYCF TTDGQMIQVD TAFQFPNGIA VRHMNDGRPY QLIVAETPTK KLWSYDIKGP AKIENKKVWG HIPGTHEGGA DGMDFDEDNN LLVANWGSSH IEVFGPDGGQ PKMRIRCPFE KPSNLHFKPQ TKTIFVTEHE NNAVWKFEWQ RNGKKQYCET LKFGIF

2. CA (non-polymer), 40.078 × 2 Da

Total weight

35303.645 Da

Max. entity weight

35223.49 Da

Source organism

Loligo vulgaris

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

780 J values in 4 lists
Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Pressure 1 atm, Temperature 301 K, pH 6.5 Detail

---

Pressure 1 atm, Temperature 301 K, pH 6.5

Experiment name 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 3D Ca-coupled HA(CACO)NH, 3D Ha-coupled [15N,1H]-TROSY-HNCA, 3D Ha-coupled [15N,1H]-TROSY-iHNCA, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA, 3D Ha-coupled [15N,1H]-TROSY-HN(CO)CA

List #1 1JCaCO, Field strength (¹H) 500 MHz

List #2 1JCaN_, Field strength (¹H) 800 MHz

List #3 1JCaHa, Field strength (¹H) 600 MHz, Details 14-ms variable-time Ca evolution in 3D TROSY experiments

List #4 1JCaCb, Field strength (¹H) 800 MHz, Details Ca evolution time typically 70-90ms

Release date

2008-11-18

Citation 1

Variation in protein C(alpha)-related one-bond J couplings
Schmidt, J.M., Howard, M.J., Maestre-Martinez, M., Perez, C.S., Lohr, F.
Magn. Reson. Chem. (2009), 47, 16-30, PubMed 18853398 , DOI 10.1002/mrc.2337 , Abstract

Show more 13 citaions

Citation 2

TROSY in triple-resonance experiments: new perspectives for sequential NMR assignment of large proteins
Salzmann, M., Pervushin, K., Wider, G., Senn, H., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (1998), 95, 13585-13590, PubMed 9811843 , Abstract

Citation 3

Recording heteronuclear quantitative J-correlation spectra with internal reference peaks
Lohr, F., Perez, C., Schmidt, J.M., Ruterjans, H.
Bull. Magn. Reson. (1999), 20, 9-14

Citation 4

TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins
Salzmann, M., Wider, G., Pervushin, K., Senn, H., Wuthrich, K.
J. Am. Chem. Soc. (1999), 121, 844-848, DOI 10.1021/ja9834226

Citation 5

TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins
Yang, D., Venters, R.A., Mueller, G.A., Choy, W.Y., Kay, L.E.
J. Biomol. NMR (1999), 14, 333-343

Citation 6

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 7

Measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in (15)N,(13)C,(2)H-labeled proteins: application to domain orientation in maltose binding protein
Evenas, J., Mittermaier, A., Yang, D., Kay, L.E.
J. Am. Chem. Soc. (2001), 123, 2858-2864, PubMed 11456973 , DOI: , Abstract

Citation 8

Intraresidue HNCA and COHNCA experiments for protein backbone resonance assignment
Brutscher, B.
J. Magn. Reson. (2002), 156, 155-159, PubMed 12081454 , Abstract

Citation 9

A novel approach for the sequential backbone assignment of larger proteins: selective intra-HNCA and DQ-HNCA
Nietlispach, D., Ito, Y., Laue, E.D.
J. Am. Chem. Soc. (2002), 124, 11199-11207, PubMed 12224968 , DOI: , Abstract

Citation 10

Intraresidual HNCA: an experiment for correlating only intraresidual backbone resonances
Permi, P.
J. Biomol. NMR (2002), 23, 201-209, PubMed 12238592 , Abstract

Citation 11

A high sensitivity 3D experiment for measuring Calpha-Halpha residual dipolar coupling constants
Hu, W., Zhang, Z., Chen, Y.
J. Magn. Reson. (2003), 165, 248-252, PubMed 14643706 , DOI 10.1016/j.jmr.2003.08.008 , Abstract

Citation 12

Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract

Citation 13

Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract

Citation 14

Measurement of one-bond 13Calpha-1Halpha residual dipolar coupling constants in proteins by selective manipulation of CalphaHalpha spins
Ball, G., Meenan, N., Bromek, K., Smith, B.O., Bella, J., Uhrin, D.
J. Magn. Reson. (2006), 180, 127-136, PubMed 16495100 , DOI 10.1016/j.jmr.2006.01.017 , Abstract

Hide non-primary 13 citaions

Entries sharing articles BMRB: 13 entries Detail

Related entities 1. DFPase, : 1 : 12 : 23 entities Detail

Experiments performed 8 experiments Detail