BMRB: 16579

2J coupling constants in oxidized Flavodoxin from Desulfovibrio vulgaris (Hildenborough)

Authors

Schmidt, J.M., Lohr, F.

Assembly

Flavodoxin oxidized

Entity

1. Flavodoxin (polymer, Thiol state: all free), 147 monomers, 15692.04 Da Detail

PKALIVYGST TGNTEYTAET IARELADAGY EVDSRDAASV EAGGLFEGFD LVLLGCSTWG DDSIELQDDF IPLFDSLEET GAQGRKVACF GCGDSSYEYF CGAVDAIEEK LKNLGAEIVQ DGLRIDGDPR AARDDIVGWA HDVRGAI

2. FMN (non-polymer, Thiol state: not present), 456.344 Da

Total weight

16148.384 Da

Max. entity weight

15692.04 Da

Source organism

Desulfovibrio vulgaris str. Hildenborough

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

536 J values in 4 lists
Field strength (¹H) 700 MHz, 800 MHz, Pressure 1 atm, Temperature 300 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 300 K, pH 7.0

Experiment name 3D Ha-coupled ct-[15N,1H]-TROSY-iHNCA, 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY

List #1 2JCaN_, Field strength (¹H) 800 MHz

List #2 2JHNCa, Field strength (¹H) 800 MHz

List #3 2JCOHN, Field strength (¹H) 800 MHz

List #4 2JN_Ha, Field strength (¹H) 700 MHz

Release date

2010-02-07

Citation 1

Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract

Show more 3 citaions

Citation 2

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 3

Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract

Citation 4

Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract

Hide non-primary 3 citaions

Related entities 1. Flavodoxin, : 1 : 1 : 4 : 19 : 176 entities Detail

Experiments performed 5 experiments Detail