BMRB: 16583

2J coupling constants in Xylanase from Bacillus agaradhaerens

Authors

Schmidt, J.M., Lohr, F.

Assembly

Xylanase

Entity

1. Xylanase (polymer, Thiol state: all free), 207 monomers, 23154.44 Da Detail

MIVTDNSIGN HDGYDYEFWK DSGGSGTMIL NHGGTFSAQW NNVNNILFRK GKKFNETQTH QQVGNMSINY GANFQPNGNA YLCVYGWTVD PLVEYYIVDS WGNWRPPGAT PKGTITVDGG TYDIYETLRV NQPSIKGIAT FKQYWSVRRS KRTSGTISVS NHFRAWENLG MNMGKMYEVA LTVEGYQSSG SANVYSNTLR INGNPLS

Formula weight

23154.44 Da

Source organism

Salipaludibacillus agaradhaerens

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

977 J values in 6 lists
Field strength (¹H) 950 MHz, 900 MHz, Pressure 1 atm, Temperature 302 K, pH 5.4 Detail

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Pressure 1 atm, Temperature 302 K, pH 5.4

Experiment name 2D CO-coupled ct-[13C,1H]-HSQC, 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 3D Ha-coupled [15N,1H]-TROSY-iHNCA, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 2D Cb-coupled [13C,1H]-HSQC

List #1 2JCaN_, Field strength (¹H) 950 MHz

List #2 2JHNCa, Field strength (¹H) 950 MHz

List #3 2JCOHN, Field strength (¹H) 950 MHz

List #4 2JN_Ha, Field strength (¹H) 950 MHz

List #5 2JHaCO, Field strength (¹H) 900 MHz

List #6 2JHaCb, Field strength (¹H) 950 MHz

Release date

2010-02-07

Citation 1

Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract

Show more 4 citaions

Citation 2

Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C
Vuister, G.W., Bax, A.
J. Biomol. NMR (1992), 2, 401-405, PubMed 1511238 , DOI 10.1007/bf01874818 , Abstract

Citation 3

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 4

Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract

Citation 5

Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract

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Entries sharing articles BMRB: 12 entries Detail

Related entities 1. Xylanase, : 1 : 4 : 160 entities Detail

Experiments performed 7 experiments Detail