BMRB: 16584

2J coupling constants in DFPase from Loligo vulgaris

Authors

Schmidt, J.M., Lohr, F.

Assembly

Diisopropylfluorophosphatase

Entity

1. DFPase (polymer, Thiol state: all free), 316 monomers, 35223.49 Da Detail

GSMEIPVIEP LFTKVTEDIP GAEGPVFDKN GDFYIVAPEV EVNGKPAGEI LRIDLKTGKK TVICKPEVNG YGGIPAGCQC DRDANQLFVA DMRLGLLVVQ TDGTFEEIAK KDSEGRRMQG CNDCAFDYEG NLWITAPAGE VAPADYTRSM QEKFGSIYCF TTDGQMIQVD TAFQFPNGIA VRHMNDGRPY QLIVAETPTK KLWSYDIKGP AKIENKKVWG HIPGTHEGGA DGMDFDEDNN LLVANWGSSH IEVFGPDGGQ PKMRIRCPFE KPSNLHFKPQ TKTIFVTEHE NNAVWKFEWQ RNGKKQYCET LKFGIF

2. CA (non-polymer, Thiol state: not present), 40.078 × 2 Da

Total weight

35303.645 Da

Max. entity weight

35223.49 Da

Source organism

Loligo vulgaris

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

796 J values in 4 lists
Field strength (¹H) 600 MHz, 800 MHz, Pressure 1 atm, Temperature 301 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 301 K, pH 6.5

Experiment name 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 3D Ha-coupled [15N,1H]-TROSY-iHNCA, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY

List #1 2JCaN_, Field strength (¹H) 800 MHz

List #2 2JHNCa, Field strength (¹H) 800 MHz

List #3 2JCOHN, Field strength (¹H) 800 MHz

List #4 2JN_Ha, Field strength (¹H) 600 MHz

Release date

2010-02-07

Citation 1

Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract

Show more 3 citaions

Citation 2

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 3

Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract

Citation 4

Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract

Hide non-primary 3 citaions

Entries sharing articles BMRB: 12 entries Detail

Related entities 1. DFPase, : 1 : 12 : 23 entities Detail

Experiments performed 5 experiments Detail