BMRB: 16962

1J and 2J coupling constants in human oxidized ERp18

Authors

Schmidt, J.M., Lohr, F.

Assembly

ERp18

Entity

1. ERp18 (polymer, Thiol state: all disulfide bound), 157 monomers, 17770.70 Da Detail

MHHHHHHMSD GHNGLGKGFG DHIHWRTLED GKKEAAASGL PLMVIIHKSW CGACKALKPK FAESTEISEL SHNFVMVNLE DEEEPKDEDF SPDGGYIPRI LFLDPSGKVH PEIINENGNP SYKYFYVSAE QVVQGMKEAQ ERLTGDAFRK KHLEDEL

Formula weight

17770.7 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS51:SG	1:CYS54:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

1268 J values in 10 lists
Field strength (¹H) 500 MHz, 800 MHz, 900 MHz, Pressure 1 atm, Temperature 303 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 303 K, pH 6.5

Experiment name 3D Ca-coupled [15N,1H]-TROSY-HNCO, 3D [1Ha,13Ca]-multiple-quantum HCAN quantitative J-correlation, 3D Ca-coupled H(CACO)NH, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 3D Ha-coupled [15N,1H]-TROSY-H(N)COCA, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 2D ct-[13C,1H]-IPAP-HSQC, 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA, 2D [15N,1H]-IPAP-HSQC, 3D Ha-coupled [15N,1H]-TROSY-HN(CO)CA

List #1 1JCaCO, Field strength (¹H) 500 MHz

List #2 1JCaN_, Field strength (¹H) 900 MHz, Details MQ-HCAN experiment for proline residues, IPAP-TROSY experiment for all other

List #3 1JCaHa, Field strength (¹H) 900 MHz

List #4 1JCaCb, Field strength (¹H) 800 MHz, Details recorded at 800 and 900 MHz with Ca evolution times of 46 and 44 ms, resp.

List #5 1JCON_, Field strength (¹H) 800 MHz

List #6 2JCaN_, Field strength (¹H) 900 MHz

List #7 2JCOHN, Field strength (¹H) 800 MHz

List #8 2JHaCO, Field strength (¹H) 500 MHz

List #9 2JHNCa, Field strength (¹H) 900 MHz

List #10 1JN_HN, Field strength (¹H) 500 MHz

Release date

2011-01-18

Citation 1

One-bond and two-bond J couplings help annotate protein secondary-structure motifs: J-coupling indexing applied to human endoplasmic reticulum protein ERp18
Schmidt, J.M., Zhou, S., Rowe, M.L., Howard, M.J., Williamson, R.A., Lohr, F.
Proteins (2011), 79, 428-443, PubMed 21117079 , DOI 10.1002/prot.22893 , Abstract

Show more 8 citaions

Citation 2

Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra
Ottiger, M., Delaglio, F., Bax, A.
J. Magn. Reson. (1998), 131, 373-378, PubMed 9571116 , DOI 10.1006/jmre.1998.1361 , Abstract

Citation 3

Measurement of (1)J(NC') and (2)J(H(N))(C') couplings from spin-state-selective two-dimensional correlation spectrum
Permi, P., Heikkinen, S., Kilpelainen, I., Annila, A.
J. Magn. Reson. (1999), 140, 32-40, PubMed 10479547 , DOI 10.1006/jmre.1999.1817 , Abstract

Citation 4

TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins
Yang, D., Venters, R.A., Mueller, G.A., Choi, W.Y., Kay, L.E.
J. Biomol. NMR (1999), 14, 333-343

Citation 5

Large variations in one-bond 13Ca-13Cb J couplings in polypeptides correlate with backbone conformation
Cornilescu, G., Bax, A.M., Case, D.A.
J. Am. Chem. Soc. (2000), 122, 2168-2171, DOI 10.1021/ja993609c

Citation 6

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 7

A high sensitivity 3D experiment for measuring Calpha-Halpha residual dipolar coupling constants
Hu, W., Zhang, Z., Chen, Y.
J. Magn. Reson. (2003), 165, 248-252, PubMed 14643706 , DOI 10.1016/j.jmr.2003.08.008 , Abstract

Citation 8

Measurement of one-bond 13Calpha-1Halpha residual dipolar coupling constants in proteins by selective manipulation of CalphaHalpha spins
Ball, G., Meenan, N., Bromek, K., Smith, B.O., Bella, J., Uhrin, D.
J. Magn. Reson. (2006), 180, 127-136, PubMed 16495100 , DOI 10.1016/j.jmr.2006.01.017 , Abstract

Citation 9

Optimum spin-state selection for all multiplicities in the acquisition dimension of the HSQC experiment
Nolis, P., Espinosa, J.F., Parella, T.
J. Magn. Reson. (2006), 180, 39-50, PubMed 16448830 , DOI 10.1016/j.jmr.2006.01.003 , Abstract

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Entries sharing articles BMRB: 13 entries Detail

Related entities 1. ERp18, : 1 : 2 : 34 entities Detail

Interaction partners 1. ERp18, : 6 interactors Detail

Experiments performed 10 experiments Detail