Backbone 1H, 13C, and 15N chemical shift assignments for the nucleotide-binding domain of E.coli DnaK in the ADP.Pi-bound state
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGAF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIELL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 45.7 % (1998 of 4374) | 24.4 % (553 of 2267) | 64.9 % (1104 of 1701) | 84.0 % (341 of 406) |
Backbone | 77.4 % (1777 of 2296) | 50.4 % (400 of 793) | 91.7 % (1037 of 1131) | 91.4 % (340 of 372) |
Sidechain | 21.9 % (533 of 2433) | 10.4 % (153 of 1474) | 41.0 % (379 of 925) | 2.9 % (1 of 34) |
Aromatic | 10.8 % (19 of 176) | 14.8 % (13 of 88) | 6.9 % (6 of 87) | 0.0 % (0 of 1) |
Methyl | 21.8 % (108 of 496) | 15.7 % (39 of 248) | 27.8 % (69 of 248) |
1. the nucleotide-binding domain of DnaK
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGAF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIELL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 (±0.2) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | potassium phosphate | natural abundance | 10 mM | |
2 | magnesium chloride | natural abundance | 10 mM | |
3 | AEBSF protease inhibitor | natural abundance | 1 uM | |
4 | DTT | natural abundance | 5 mM | |
5 | DSS | natural abundance | 0.5 mM | |
6 | ADP | natural abundance | 10 mM | |
7 | potassium chloride | natural abundance | 10 mM | |
8 | the nucleotide-binding domain of DnaK | [U-97% 2H; U-95% 13C; U-95% 15N] | 0.5 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |