Backbone 1H, 13C, and 15N chemical shift assignments for the nucleotide-binding domain of E.coli DnaK in the nucleotide-free state
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGAF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIELL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 42.0 % (1835 of 4374) | 27.7 % (627 of 2267) | 51.1 % (870 of 1701) | 83.3 % (338 of 406) |
Backbone | 67.3 % (1545 of 2296) | 52.2 % (414 of 793) | 70.2 % (794 of 1131) | 90.6 % (337 of 372) |
Sidechain | 15.4 % (374 of 2433) | 14.1 % (208 of 1474) | 17.8 % (165 of 925) | 2.9 % (1 of 34) |
Aromatic | 14.8 % (26 of 176) | 14.8 % (13 of 88) | 14.9 % (13 of 87) | 0.0 % (0 of 1) |
Methyl | 16.5 % (82 of 496) | 16.1 % (40 of 248) | 16.9 % (42 of 248) |
1. the nucleotide-binding domain of DnaK
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGAF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIELL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 299 (±0.2) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | potassium phosphate | natural abundance | 10 mM | |
2 | magnesium chloride | natural abundance | 10 mM | |
3 | AEBSF protease inhibitor | natural abundance | 1 uM | |
4 | DTT | natural abundance | 5 mM | |
5 | DSS | natural abundance | 0.5 mM | |
6 | potassium chloride | natural abundance | 10 mM | |
7 | the nucleotide-binding domain of DnaK | [U-97% 2H; U-95% 13C; U-95% 15N] | 0.5 mM | |
8 | H2O | natural abundance | 95 % | |
9 | D2O | natural abundance | 5 % |