BMRBj - BMREntryMaster

Found 1 Document (5.066 seconds)

BMRB: 17604

2LCB

Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant

Authors

Bouvignies, G., Vallurupalli, P., Hansen, D., Correia, B., Lange, O., Bah, A., Vernon, R.M., Dahlquist, F.W., Baker, D., Kay, L.E.

Assembly

T4 L99A

Entity

1. T4 L99A (polymer, Thiol state: not present), 164 monomers, 18559.05 Da Detail

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Formula weight

18559.05 Da

Source organism

Escherichia virus T4

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 37.9 %, Completeness (bb): 71.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.9 % (734 of 1935)	29.6 % (298 of 1008)	37.3 % (278 of 746)	87.3 % (158 of 181)
Backbone	71.4 % (698 of 978)	79.8 % (268 of 336)	56.5 % (272 of 481)	98.1 % (158 of 161)
Sidechain	4.3 % (48 of 1110)	4.5 % (30 of 672)	4.3 % (18 of 418)	0.0 % (0 of 20)
Aromatic	0.0 % (0 of 138)	0.0 % (0 of 69)	0.0 % (0 of 66)	0.0 % (0 of 3)
Methyl	3.1 % (6 of 194)	4.1 % (4 of 97)	2.1 % (2 of 97)

1. T4 L99A

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
8	T4 L99A	[U-13C; U-15N; U-2H]	1.5 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaCl	natural abundance	25 mM
12	EDTA	natural abundance	2 mM
13	NaN3	natural abundance	2 mM
14	sodium phosphate	natural abundance	50 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
15	T4 L99A	[U-13Ca; U-15N]	1.5 mM
16	D2O	natural abundance	100 %
17	NaCl	natural abundance	25 mM
18	EDTA	natural abundance	2 mM
19	NaN3	natural abundance	2 mM
20	sodium phosphate	natural abundance	50 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
27	T4 L99A	[ U-15N] 13CH3 Met	1.5 mM
28	D2O	natural abundance	100 %
29	NaCl	natural abundance	25 mM
30	EDTA	natural abundance	2 mM
31	NaN3	natural abundance	2 mM
32	sodium phosphate	natural abundance	50 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2LCB, Strand ID: A Detail

Release date

2011-04-28

Citation

Solution structure of a minor and transiently formed state of a T4 lysozyme mutant
Bouvignies, G., Vallurupalli, P., Hansen, D., Correia, B.E., Lange, O., Bah, A., Vernon, R.M., Dahlquist, F.W., Baker, D., Kay, L.E.
Nature (2011), 477, 111-114, PubMed 21857680 , DOI 10.1038/nature10349 , Abstract

Related entities 1. T4 L99A, : 1 : 35 : 66 : 11 entities Detail

Experiments performed 29 experiments Detail

1 15N CT Trosy CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

2 15N CT Trosy CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

3 1H CT CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

4 1H CT CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

5 1H ZQ/DQ CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

6 1H ZQ/DQ CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

7 13C' CT CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
8	T4 L99A	[U-13C; U-15N; U-2H]	1.5 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaCl	natural abundance	25 mM
12	EDTA	natural abundance	2 mM
13	NaN3	natural abundance	2 mM
14	sodium phosphate	natural abundance	50 mM

8 13C' CT CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
8	T4 L99A	[U-13C; U-15N; U-2H]	1.5 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaCl	natural abundance	25 mM
12	EDTA	natural abundance	2 mM
13	NaN3	natural abundance	2 mM
14	sodium phosphate	natural abundance	50 mM

9 13Ca CT CPMG

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
15	T4 L99A	[U-13Ca; U-15N]	1.5 mM
16	D2O	natural abundance	100 %
17	NaCl	natural abundance	25 mM
18	EDTA	natural abundance	2 mM
19	NaN3	natural abundance	2 mM
20	sodium phosphate	natural abundance	50 mM

10 13Ca CT CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
15	T4 L99A	[U-13Ca; U-15N]	1.5 mM
16	D2O	natural abundance	100 %
17	NaCl	natural abundance	25 mM
18	EDTA	natural abundance	2 mM
19	NaN3	natural abundance	2 mM
20	sodium phosphate	natural abundance	50 mM

11 1Ha CT CPMG

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

12 1Ha CT CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

13 Gly 13Ca CT CPMG

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

14 Gly 1Ha CT CPMG

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

15 Gly 1Ha CT CPMG

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

16 1H-15N ZZ exchange

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

17 1H-13C Met ZZ exchange

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
27	T4 L99A	[ U-15N] 13CH3 Met	1.5 mM
28	D2O	natural abundance	100 %
29	NaCl	natural abundance	25 mM
30	EDTA	natural abundance	2 mM
31	NaN3	natural abundance	2 mM
32	sodium phosphate	natural abundance	50 mM

18 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

19 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

20 2D 1H-15N HMQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

21 2D 1H-15N HMQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

22 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

23 3D HNCO

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	T4 L99A	[U-15N; U-2H]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	25 mM
5	EDTA	natural abundance	2 mM
6	NaN3	natural abundance	2 mM
7	sodium phosphate	natural abundance	50 mM

24 3D MQ HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
8	T4 L99A	[U-13C; U-15N; U-2H]	1.5 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaCl	natural abundance	25 mM
12	EDTA	natural abundance	2 mM
13	NaN3	natural abundance	2 mM
14	sodium phosphate	natural abundance	50 mM

25 3D MQ HNCO

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
8	T4 L99A	[U-13C; U-15N; U-2H]	1.5 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaCl	natural abundance	25 mM
12	EDTA	natural abundance	2 mM
13	NaN3	natural abundance	2 mM
14	sodium phosphate	natural abundance	50 mM

26 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
15	T4 L99A	[U-13Ca; U-15N]	1.5 mM
16	D2O	natural abundance	100 %
17	NaCl	natural abundance	25 mM
18	EDTA	natural abundance	2 mM
19	NaN3	natural abundance	2 mM
20	sodium phosphate	natural abundance	50 mM

27 2D 1H-13C HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
15	T4 L99A	[U-13Ca; U-15N]	1.5 mM
16	D2O	natural abundance	100 %
17	NaCl	natural abundance	25 mM
18	EDTA	natural abundance	2 mM
19	NaN3	natural abundance	2 mM
20	sodium phosphate	natural abundance	50 mM

28 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz Cryo Probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

29 2D 1H-13C HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
21	T4 L99A	[U-13C; U-15N; U-50% 2H]	1.5 mM
22	D2O	natural abundance	100 %
23	NaCl	natural abundance	25 mM
24	EDTA	natural abundance	2 mM
25	NaN3	natural abundance	2 mM
26	sodium phosphate	natural abundance	50 mM

nullKeywords Excited State, T4 Lysozyme L99A

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Found 1 Document (5.066 seconds)

BMRB: 17604

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Related entities 1. T4 L99A, : 1 : 35 : 66 : 11 entities Detail

Experiments performed 29 experiments Detail

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