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BMRB: 17845

6RSJ

Backbone 1H, 13C, and 15N chemical shift assignments of oxidized yeast iso-1 cytochrome c

Authors

Volkov, A.N., Van de Water, K., Vanwetswinkel, S., van Nuland, N.A. J.

Assembly

yCc

Entity

1. yCc (polymer, Thiol state: all other bound), 108 monomers, 12018.58 Da Detail

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

2. HEC (non-polymer), 618.503 Da

Total weight

12637.083 Da

Max. entity weight

12018.58 Da

Entity Connection

na 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS19:SG	2:HEC1:HEC
2	na	sing	1:CYS22:SG	2:HEC1:HEC

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 44.1 %, Completeness (bb): 79.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.1 % (562 of 1274)	21.2 % (142 of 670)	63.1 % (309 of 490)	97.4 % (111 of 114)
Backbone	79.8 % (511 of 640)	47.8 % (107 of 224)	96.8 % (302 of 312)	98.1 % (102 of 104)
Sidechain	20.0 % (146 of 730)	7.8 % (35 of 446)	37.2 % (102 of 274)	90.0 % (9 of 10)
Aromatic	0.9 % (1 of 108)	1.9 % (1 of 54)	0.0 % (0 of 53)	0.0 % (0 of 1)
Methyl	9.8 % (9 of 92)	2.2 % (1 of 46)	17.4 % (8 of 46)

1. yCc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	yCc	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	yCc	[U-100% 13C; U-100% 15N]	1 mM
7	sodium phosphate	natural abundance	20 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Chem. Shift Complete2

Sequence coverage: 94.4 %, Completeness: 33.6 %, Completeness (bb): 57.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.6 % (857 of 2548)	22.0 % (295 of 1340)	34.9 % (342 of 980)	96.5 % (220 of 228)
Backbone	57.8 % (740 of 1280)	48.0 % (215 of 448)	51.8 % (323 of 624)	97.1 % (202 of 208)
Sidechain	14.9 % (217 of 1460)	8.9 % (79 of 892)	21.9 % (120 of 548)	90.0 % (18 of 20)
Aromatic	4.2 % (9 of 216)	4.6 % (5 of 108)	3.8 % (4 of 106)	0.0 % (0 of 2)
Methyl	9.2 % (17 of 184)	5.4 % (5 of 92)	13.0 % (12 of 92)

1. yCc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	yCc	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	yCc	[U-100% 13C; U-100% 15N]	1 mM
7	sodium phosphate	natural abundance	20 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Release date

2012-03-13

Citation

Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy
Volkov, A.N., Vanwetswinkel, S., Van de Water, K., van Nuland, N.A. J.
J. Biomol. NMR (2012), 52, 245-256, PubMed 22318343 , DOI 10.1007/s10858-012-9607-8 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 17846 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of reduced yeast iso-1 cytochrome c
  BMRB: 17847 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of oxidized horse heart cytochrome c
  BMRB: 17848 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of reduced horse heart cytochrome c

Related entities 1. yCc, : 1 : 22 : 45 : 169 entities Detail

Interaction partners 1. yCc, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC