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Backbone 1H, 13C, and 15N chemical shift assignments of reduced horse heart cytochrome c
Authors
Volkov, A.N., Van de Water, K., Vanwetswinkel, S., van Nuland, N.A. J.
Assembly
hCc
Entity
1. hCc (polymer, Thiol state: all other bound), 104 monomers, 11701.40 Da Detail

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE


2. HEC (non-polymer), 618.503 Da
Total weight
12319.903 Da
Max. entity weight
11701.4 Da
Entity Connection
na 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS14:SG2:HEC1:HEC
2nasing1:CYS17:SG2:HEC1:HEC

Source organism
Equus caballus
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 40.9 %, Completeness (bb): 78.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All40.9 % (516 of 1262)17.8 % (119 of 667)60.3 % (292 of 484)94.6 % (105 of 111)
Backbone78.3 % (473 of 604)43.1 % (88 of 204)96.0 % (288 of 300)97.0 % (97 of 100)
Sidechain17.9 % (134 of 750) 6.7 % (31 of 463)34.4 % (95 of 276)72.7 % (8 of 11)
Aromatic 0.0 % (0 of 96) 0.0 % (0 of 48) 0.0 % (0 of 47) 0.0 % (0 of 1)
Methyl 6.5 % (6 of 92) 0.0 % (0 of 46)13.0 % (6 of 46)

1. hCc

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
1hCc[U-100% 13C; U-100% 15N]1 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance100 mM
4H2Onatural abundance95 %
5D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
6hCc[U-100% 13C; U-100% 15N]1 mM
7sodium phosphatenatural abundance20 mM
8H2Onatural abundance95 %
9D2Onatural abundance5 %

Chem. Shift Complete2
Sequence coverage: 94.2 %, Completeness: 32.2 %, Completeness (bb): 57.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All32.2 % (813 of 2524)20.5 % (274 of 1334)34.0 % (329 of 968)94.6 % (210 of 222)
Backbone57.3 % (692 of 1208)45.3 % (185 of 408)52.2 % (313 of 600)97.0 % (194 of 200)
Sidechain14.8 % (222 of 1500) 9.6 % (89 of 926)21.2 % (117 of 552)72.7 % (16 of 22)
Aromatic 0.0 % (0 of 192) 0.0 % (0 of 96) 0.0 % (0 of 94) 0.0 % (0 of 2)
Methyl 8.7 % (16 of 184) 5.4 % (5 of 92)12.0 % (11 of 92)

1. hCc

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
1hCc[U-100% 13C; U-100% 15N]1 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance100 mM
4H2Onatural abundance95 %
5D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
6hCc[U-100% 13C; U-100% 15N]1 mM
7sodium phosphatenatural abundance20 mM
8H2Onatural abundance95 %
9D2Onatural abundance5 %

Release date
2012-03-13
Citation
Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy
Volkov, A.N., Vanwetswinkel, S., Van de Water, K., van Nuland, N.A. J.
J. Biomol. NMR (2012), 52, 245-256, PubMed 22318343 , DOI 10.1007/s10858-012-9607-8 ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 17845 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of oxidized yeast iso-1 cytochrome c
  BMRB: 17846 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of reduced yeast iso-1 cytochrome c
  BMRB: 17847 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of oxidized horse heart cytochrome c
Related entities 1. hCc, : 1 : 27 : 2 : 1 : 169 entities Detail
Interaction partners 1. hCc, : 1 interactors Detail
Experiments performed 6 experiments Detail