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Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the absence of Ca2+
Authors
Wang, X., Kleerekoper, Q.K., Putkey, J.A.
Assembly
C-CaM/PEP-19 complex
Entity
1. isolcated c domain of calmodulin (polymer, Thiol state: not present), 71 monomers, 8147.759 Da Detail

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K


2. PEP-19 (polymer, Thiol state: not present), 62 monomers, 6775.333 Da Detail

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS


Total weight
14923.092 Da
Max. entity weight
8147.759 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation
Chem. Shift Complete
Sequence coverage: 52.6 %, Completeness: 16.0 %, Completeness (bb): 34.8 % Detail

Polymer type: polypeptide(L)

Total1H15N
All16.0 % (150 of 939)10.1 % (79 of 786)46.4 % (71 of 153)
Backbone34.8 % (138 of 397)25.7 % (68 of 265)53.0 % (70 of 132)
Sidechain 2.2 % (12 of 542) 2.1 % (11 of 521) 4.8 % (1 of 21)
Aromatic 0.0 % (0 of 40) 0.0 % (0 of 40)
Methyl 3.8 % (2 of 52) 3.8 % (2 of 52)

1. isolcated c domain of calmodulin

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. PEP-19

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 15N labeled apo isolated C-domain of CaM in complex with PEP-19


#NameIsotope labelingTypeConcentration
1isolcated c domain of calmodulin[U-100% 15N]0.5 mM
2PEP-19natural abundance0.8 mM
3EDTAnatural abundance10 mM
4potassium chloridenatural abundance100 mM
5H2Onatural abundance95 %
6D2Onatural abundance5 %

Heteronucl. T1
62 T1 values in 1 lists
Coherence Sz, Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. T2
63 T2 values in 1 lists
Coherence S(+,-), Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. NOE
63 NOE values in 1 lists
Value type relative intensities, Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. T1/T2
62 T1/T2 values in 1 lists
Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Release date
2011-10-09
Citation
Intrinsically disordered PEP-19 confers unique dynamic properties to apo and calcium calmodulin
Wang, X., Kleerekoper, Q.K., Xiong, L., Putkey, J.A.
Biochemistry (2010), 49, 10287-10297, PubMed 20973509 , DOI 10.1021/bi100500m ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 17881 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin apo form
  BMRB: 17982 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with Ca2+
  BMRB: 17983 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the presence of Ca2+
Related entities 2. PEP-19, : 1 : 6 entities Detail
Interaction partners 2. PEP-19, : 5 interactors Detail
Keywords assignment, backbone dynamics, calcium binding, calmodulin, PEP-19