BMRB: 17983

Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the presence of Ca2+

Authors

Wang, X., Kleerekoper, Q.K., Putkey, J.A.

Assembly

C-CaM/PEP-19 complex

Entity

1. isolcated c domain of calmodulin (polymer, Thiol state: not present), 71 monomers, 8147.759 Da Detail

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. PEP-19 (polymer, Thiol state: not present), 62 monomers, 6775.333 Da Detail

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS

3. CA (non-polymer), 40.078 Da

Total weight

14963.17 Da

Max. entity weight

8147.759 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 50.4 %, Completeness: 15.8 %, Completeness (bb): 33.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	15.8 % (147 of 932)	10.3 % (81 of 786)	45.2 % (66 of 146)
Backbone	33.6 % (137 of 408)	25.7 % (71 of 276)	50.0 % (66 of 132)
Sidechain	2.1 % (11 of 524)	2.0 % (10 of 510)	7.1 % (1 of 14)
Aromatic	0.0 % (0 of 40)	0.0 % (0 of 40)
Methyl	0.0 % (0 of 52)	0.0 % (0 of 52)

1. isolcated c domain of calmodulin

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. PEP-19

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 15N labeled isolated C-domain of CaM in complex with PEP-19

#	Name	Isotope labeling	Type	Concentration
1	isolcated c domain of calmodulin	natural abundance		0.5 mM
2	PEP-19	natural abundance		0.8 mM
3	calcium	natural abundance		5 mM
4	potassium chloride	natural abundance		100 mM
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

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Heteronucl. T₁

63 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name T1

Heteronucl. T₂

64 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name T2

Heteronucl. NOE

63 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name NOE

Heteronucl. T₁/T₂

62 T₁/T₂ values in 1 lists
Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Release date

2011-10-09

Citation

Intrinsically disordered PEP-19 confers unique dynamic properties to apo and calcium calmodulin
Wang, X., Kleerekoper, Q.K., Xiong, L., Putkey, J.A.
Biochemistry (2010), 49, 10287-10297, PubMed 20973509 , DOI 10.1021/bi100500m , Abstract

Related entities 1. isolcated c domain of calmodulin, : 11 : 174 entities Detail

Related entities 2. PEP-19, : 2 : 12 entities Detail

Interaction partners 2. PEP-19, : 5 interactors Detail

Experiments performed 11 experiments Detail

Chemical shift validation 5 contents Detail