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BMRB: 18933

2M2T

ASFV Pol X structure

Authors

Wu, W., Su, M., Tsai, M.

Assembly

ASFV Pol X

Entity

1. ASFV Pol X (polymer, Thiol state: all free), 174 monomers, 20314.16 Da Detail

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

Formula weight

20314.16 Da

Source organism

African swine fever virus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 37.3 %, Completeness (bb): 73.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.3 % (834 of 2238)	18.7 % (220 of 1179)	52.0 % (456 of 877)	86.8 % (158 of 182)
Backbone	73.5 % (759 of 1032)	48.0 % (168 of 350)	84.6 % (435 of 514)	92.9 % (156 of 168)
Sidechain	16.2 % (222 of 1372)	6.3 % (52 of 829)	31.8 % (168 of 529)	14.3 % (2 of 14)
Aromatic	0.0 % (0 of 160)	0.0 % (0 of 80)	0.0 % (0 of 79)	0.0 % (0 of 1)
Methyl	11.8 % (29 of 246)	11.4 % (14 of 123)	12.2 % (15 of 123)

1. entity

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N; U-80% 2H]	1.0 mM
2	DTT	[U-2H]	10 mM
3	potassium chloride	natural abundance	50 mM
4	borate	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	entity	[U-100% 13C; U-100% 15N]	1.0 mM
6	DTT	[U-2H]	10 mM
7	potassium chloride	natural abundance	50 mM
8	borate	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.69 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.69 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M2T, Strand ID: A Detail

Release date

2014-03-30

Citation

How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation
Wu, W., Su, M., Wu, J., Kumar, S., Lim, L., Wang, C., Nelissen, F.H.T., Chen, M., Doreleijers, J.F., Wijmenga, S.S., Tsai, M.
J. Am. Chem. Soc. (2014), 136, 4927-4937, PubMed 24617852 , DOI 10.1021/ja4102375 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 18934 released on 2014-03-30
    Title Binary complex of African Swine Fever Virus Pol X with MgdGTP
  BMRB: 18935 released on 2014-03-30
    Title African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA

Related entities 1. ASFV Pol X, : 1 : 20 : 2 : 3 : 104 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC