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BMRB: 18934

2M2U

Binary complex of African Swine Fever Virus Pol X with MgdGTP

Authors

Wu, W., Su, M., Tsai, M.

Assembly

Binary complex of African Swine Fever Virus Pol X with MgdGTP

Entity

1. Binary complex of African Swine Fever Virus Pol X with MgdGTP, entity 1 (polymer, Thiol state: all free), 174 monomers, 20314.16 Da Detail

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

2. Binary complex of African Swine Fever Virus Pol X with MgdGTP, entity MG (non-polymer), 24.305 × 2 Da
3. Binary complex of African Swine Fever Virus Pol X with MgdGTP, entity DGT (non-polymer), 507.181 Da

Total weight

20869.951 Da

Max. entity weight

20314.16 Da

Source organism

African swine fever virus

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.6 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.6 % (2117 of 2238)	96.5 % (1138 of 1179)	91.6 % (803 of 877)	96.7 % (176 of 182)
Backbone	97.5 % (1006 of 1032)	98.9 % (346 of 350)	95.9 % (493 of 514)	99.4 % (167 of 168)
Sidechain	93.1 % (1277 of 1372)	95.5 % (792 of 829)	90.0 % (476 of 529)	64.3 % (9 of 14)
Aromatic	78.8 % (126 of 160)	97.5 % (78 of 80)	59.5 % (47 of 79)	100.0 % (1 of 1)
Methyl	99.6 % (245 of 246)	99.2 % (122 of 123)	100.0 % (123 of 123)

1. entity 1

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	African Swine Fever Virus Pol X	[U-100% 13C; U-100% 15N]	0.5 mM
2	2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	[U-100% 13C; U-100% 15N]	5 mM
3	MG2	natural abundance	10 mM
4	DTT	[U-100% 2H]	10 mM
5	Borate	natural abundance	50 mM
6	potassium chloride	natural abundance	50 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	African Swine Fever Virus Pol X	[U-13C; U-15N; U-2H]	0.5 mM
8	2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	natural abundance	5 mM
9	MG2	natural abundance	10 mM
10	DTT	[U-100% 2H]	10 mM
11	Borate	natural abundance	50 mM
12	potassium chloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.76 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.76 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 3.09 ppm, Outliers: 8 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M2U, Strand ID: A Detail

Release date

2014-03-30

Citation

How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation
Wu, W., Su, M., Wu, J., Kumar, S., Lim, L., Wang, C., Nelissen, F.H.T., Chen, M., Doreleijers, J.F., Wijmenga, S.S., Tsai, M.
J. Am. Chem. Soc. (2014), 136, 4927-4937, PubMed 24617852 , DOI 10.1021/ja4102375 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 18933 released on 2014-03-30
    Title ASFV Pol X structure
  BMRB: 18935 released on 2014-03-30
    Title African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA

Related entities 1. Binary complex of African Swine Fever Virus Pol X with MgdGTP, entity 1, : 1 : 20 : 2 : 3 : 104 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC