Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli
AGNKPFNKQQ AEPRERDPQV AGLKVPPHSI EAEQSVLGGL MLDNERWDDV AERVVADDFY TRPHRHIFTE MARLQESGSP IDLITLAESL ERQGQLDSVG GFAYLAELSK NTPSAANISA YADIVRERAV VREMISVANE IA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 45.4 % (729 of 1604) | 37.1 % (310 of 835) | 47.3 % (294 of 621) | 84.5 % (125 of 148) |
Backbone | 79.4 % (664 of 836) | 91.9 % (261 of 284) | 66.7 % (279 of 418) | 92.5 % (124 of 134) |
Sidechain | 9.4 % (85 of 902) | 8.9 % (49 of 551) | 10.7 % (36 of 337) | 0.0 % (0 of 14) |
Aromatic | 0.0 % (0 of 88) | 0.0 % (0 of 44) | 0.0 % (0 of 43) | 0.0 % (0 of 1) |
Methyl | 13.0 % (21 of 162) | 12.3 % (10 of 81) | 13.6 % (11 of 81) |
1. DnaB helicase
AGNKPFNKQQ AEPRERDPQV AGLKVPPHSI EAEQSVLGGL MLDNERWDDV AERVVADDFY TRPHRHIFTE MARLQESGSP IDLITLAESL ERQGQLDSVG GFAYLAELSK NTPSAANISA YADIVRERAV VREMISVANE IATemperature 305 (±0.1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DnaB helicase | [U-13C;U-15N] | 1.8 mM | |
2 | sodium phosphate | 20 mM | ||
3 | pefabloc | 1 mM | ||
4 | EDTA | 1 mM | ||
5 | NaN3 | 0.02 % w/v |