BMRB: 4097

Backbone 1H, 13C and 15N Chemical Shift Assignment for the HIV-1 RNase H Domain

Authors

Gunther, K., Handel, T.M., Marqusee, S.

Assembly

RNase H domain of HIV-1 RVT strain BH10

Entity

1. RNase H domain of HIV-1 RVT strain BH10 (polymer), 135 monomers, 14884.85 Da Detail

MYQLEKEPIV GAETFYVDGA ANRETKLGKA GYVTNKGRQK VVPLTNTTNQ KTELQAIYLA LQDSGLEVNI VTDSQYALGI IQAQPDKSES ELVNQIIEQL IKKEKVYLAW VPAHKGIGGN EQVDKLVSAG IRKIL

Formula weight

14884.85 Da

Source organism

Human immunodeficiency virus type 1 BH10

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 30.7 %, Completeness (bb): 56.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	30.7 % (487 of 1588)	18.1 % (150 of 827)	37.2 % (227 of 611)	73.3 % (110 of 150)
Backbone	56.7 % (455 of 802)	44.8 % (124 of 277)	56.1 % (221 of 394)	84.0 % (110 of 131)
Sidechain	14.8 % (135 of 910)	4.9 % (27 of 550)	31.7 % (108 of 341)	0.0 % (0 of 19)
Aromatic	0.0 % (0 of 74)	0.0 % (0 of 37)	0.0 % (0 of 36)	0.0 % (0 of 1)
Methyl	14.3 % (26 of 182)	12.1 % (11 of 91)	16.5 % (15 of 91)

1. Reverse Transcriptase of HIV-1

MYQLEKEPIV GAETFYVDGA ANRETKLGKA GYVTNKGRQK VVPLTNTTNQ KTELQAIYLA LQDSGLEVNI VTDSQYALGI IQAQPDKSES ELVNQIIEQL IKKEKVYLAW VPAHKGIGGN EQVDKLVSAG IRKIL

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Sample

Temperature 298 (±.5) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Reverse Transcriptase of HIV-1	[U-99.8% 13C; U-99.9% 15N]		1.1 mM

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

1999-01-24

Citation 1

Characterization of a folding intermediate from HIV-1 ribonuclease H
Gunther, K., Handel, T.M., Marqusee, S.
Protein Sci. (1998), 7, 2164-2174, PubMed 9792104 , DOI 10.1002/pro.5560071014 , Abstract

Show more 2 citaions

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Citation 3

Secondary structure of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase in solution using three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy
Powers, R., Clore, G.M., Bax, A., Garrett, D.S., Stahl, S.J., Wingfield, P.T., Gronenborn, A.M.
J. Mol. Biol. (1991), 221, 1081-1090, PubMed 1719214 , Abstract

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Related entities 1. RNase H domain of HIV-1 RVT strain BH10, : 1 : 48 : 161 entities Detail

Interaction partners 1. RNase H domain of HIV-1 RVT strain BH10, : 3 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail