1H, 15N and 13C Resonance Assignments and Secondary Structure of Apo Liver Fatty Acid-Binding Protein
MNFSGKYQVQ SQENFEPFMK AMGLPEDLIQ KGKDIKGVSE IVHEGKKVKL TITYGSKVIH NEFTLGEECE LETMTGEKVK AVVKMEGDNK MVTTFKGIKS VTEFNGDTIT NTMTLGDIVY KRVSKRI
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 79.1 % (1195 of 1511) | 74.9 % (592 of 790) | 84.0 % (492 of 586) | 82.2 % (111 of 135) |
Backbone | 92.0 % (697 of 758) | 90.5 % (239 of 264) | 94.3 % (348 of 369) | 88.0 % (110 of 125) |
Sidechain | 69.8 % (606 of 868) | 67.1 % (353 of 526) | 75.9 % (252 of 332) | 10.0 % (1 of 10) |
Aromatic | 0.0 % (0 of 92) | 0.0 % (0 of 46) | 0.0 % (0 of 46) | |
Methyl | 84.6 % (115 of 136) | 86.8 % (59 of 68) | 82.4 % (56 of 68) |
1. Liver Fatty Acid-Binding Protein
MNFSGKYQVQ SQENFEPFMK AMGLPEDLIQ KGKDIKGVSE IVHEGKKVKL TITYGSKVIH NEFTLGEECE LETMTGEKVK AVVKMEGDNK MVTTFKGIKS VTEFNGDTIT NTMTLGDIVY KRVSKRITemperature 303 (±0.1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Liver Fatty Acid-Binding Protein | [U-98% 15N] | 1.0 ~ 1.3 mM | |
10 | phosphate | 20 mM | ||
11 | D2O | 5 % | ||
12 | H2O | 95 % | ||
13 | NaCl | 100 mM | ||
14 | EDTA | 50 uM | ||
15 | sodium_azide | 0.02 % | ||
16 | bovine_lung_aprotinin | 0.01 % |