Solution Structure of the 30 Kda N-terminal Domain of Enzyme I of the Escherichia coli Phosphoenolpyruvate:Sugar Phosphotransferase System by Multidimensional NMR
MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDR
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 71.5 % (2110 of 2952) | 78.7 % (1203 of 1528) | 72.0 % (829 of 1151) | 28.6 % (78 of 273) |
Backbone | 72.0 % (1113 of 1546) | 96.0 % (508 of 529) | 70.2 % (535 of 762) | 27.5 % (70 of 255) |
Sidechain | 74.6 % (1231 of 1650) | 69.3 % (692 of 999) | 83.9 % (531 of 633) | 44.4 % (8 of 18) |
Aromatic | 44.4 % (40 of 90) | 64.4 % (29 of 45) | 24.4 % (11 of 45) | |
Methyl | 89.6 % (326 of 364) | 91.8 % (167 of 182) | 87.4 % (159 of 182) |
1. N-terminal Domain of Enzyme I
MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDRTemperature 313 (±2) K, pH 7.5 (±0.5)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal Domain of Enzyme I | [U-95% 2H; U-99% 13C; U-99% 15N] | 1.4 ~ 1.5 mM |
Temperature 313 (±2) K, pH 7.5 (±0.5)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | N-terminal Domain of Enzyme I | [U-100% 1H; U-100% 12C; U-99%1 5N] | 1.4 ~ 1.5 mM |
Temperature 313 (±2) K, pH 7.5 (±0.5)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | N-terminal Domain of Enzyme I | [U-100% 2H; U-100% 12C; U-99% 15N] | 1.4 ~ 1.5 mM |