Chemical Shift Assignments of the Mg2+-Loaded State of the N-Terminal Domain of Calmodulin
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 96.1 % (537 of 559) | 95.6 % (452 of 473) | 98.8 % (85 of 86) |
Backbone | 98.0 % (240 of 245) | 97.6 % (162 of 166) | 98.7 % (78 of 79) |
Sidechain | 94.6 % (297 of 314) | 94.5 % (290 of 307) | 100.0 % (7 of 7) |
Aromatic | 100.0 % (25 of 25) | 100.0 % (25 of 25) | |
Methyl | 97.4 % (38 of 39) | 97.4 % (38 of 39) |
1. N-terminal domain of calmodulin
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DTemperature 298 (±1) K, pH 7.2 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal domain of calmodulin | 4.0 mM | ||
2 | D2O | 10 % | ||
3 | H2O | 90 % | ||
4 | KCL | 10 mM | ||
5 | Azide | 100 uM |
Temperature 298 (±1) K, pH 7.2 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | N-terminal domain of calmodulin | [U-15N] | 4.0 mM | |
7 | D2O | 10 % | ||
8 | H2O | 90 % | ||
9 | KCL | 10 mM | ||
10 | Azide | 100 uM |