Solution Structure of Reduced Horse Heart Cytochrome c
GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | thioether | sing | 2:HEC1:CAB | 1:CYS14:SG |
2 | thioether | sing | 2:HEC1:CAC | 1:CYS17:SG |
3 | metal coordination | sing | 2:HEC1:FE | 1:HIS18:NE2 |
4 | metal coordination | sing | 2:HEC1:FE | 1:MET80:SD |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 88.6 % (591 of 667) | 88.6 % (591 of 667) |
Backbone | 98.6 % (213 of 216) | 98.6 % (213 of 216) |
Sidechain | 83.8 % (378 of 451) | 83.8 % (378 of 451) |
Aromatic | 100.0 % (48 of 48) | 100.0 % (48 of 48) |
Methyl | 95.7 % (44 of 46) | 95.7 % (44 of 46) |
1. cytochrome c
GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNEPressure 1013 mbar, Temperature 293 (±1) K, pH 7.0 (±0.1), Details The sample was lyophilized in a phosphate solution. Reduction of the protein was accomplished by addition of sodium dithionite.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c | 2.5 ~ 3.0 mM | ||
2 | phosphate | 100 mM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 47.7 % (636 of 1334) | 47.7 % (636 of 1334) |
Backbone | 54.9 % (237 of 432) | 54.9 % (237 of 432) |
Sidechain | 44.2 % (399 of 902) | 44.2 % (399 of 902) |
Aromatic | 50.0 % (48 of 96) | 50.0 % (48 of 96) |
Methyl | 52.2 % (48 of 92) | 52.2 % (48 of 92) |
1. cytochrome c
GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNEPressure 1013 mbar, Temperature 293 (±1) K, pH 7.0 (±0.1), Details The sample was lyophilized in a phosphate solution. Reduction of the protein was accomplished by addition of sodium dithionite.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c | 2.5 ~ 3.0 mM | ||
2 | phosphate | 100 mM |