An NMR Conformational Analysis of Synthetic Peptide Cn2(1-15)NH2-S-S-AcetylCn2(52-66)NH2 from the New World Centruroides Noxius 2(Cn2) Scorpion Toxin. Comparison of the Structure with those of the Centruroides Scorpion Toxins.
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS12:SG | 2:CYS14:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 50.8 % (94 of 185) | 50.8 % (94 of 185) |
Backbone | 57.1 % (32 of 56) | 57.1 % (32 of 56) |
Sidechain | 48.1 % (62 of 129) | 48.1 % (62 of 129) |
Aromatic | 57.1 % (8 of 14) | 57.1 % (8 of 14) |
Methyl | 41.7 % (5 of 12) | 41.7 % (5 of 12) |
1. Cn2(1-15)NH2
KEGYLVDKNT GCKYQX2. Ac-Cn2(52-66)NH2
XEQAIVWPLP NKRCXTemperature 298 (±0.5) K, pH 5.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cn2(1-15)NH2 | 5.0 mM | ||
2 | Ac-Cn2(52-66)NH2 | 5.0 mM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 47.8 % (177 of 370) | 47.8 % (177 of 370) |
Backbone | 50.0 % (56 of 112) | 50.0 % (56 of 112) |
Sidechain | 46.9 % (121 of 258) | 46.9 % (121 of 258) |
Aromatic | 28.6 % (8 of 28) | 28.6 % (8 of 28) |
Methyl | 50.0 % (12 of 24) | 50.0 % (12 of 24) |
1. Cn2(1-15)NH2
KEGYLVDKNT GCKYQX2. Ac-Cn2(52-66)NH2
XEQAIVWPLP NKRCXTemperature 298 (±0.5) K, pH 5.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cn2(1-15)NH2 | 5.0 mM | ||
2 | Ac-Cn2(52-66)NH2 | 5.0 mM |