BMRB: 4207

CRO Repressor Insertion Mutant K56-[DGEVK]

Authors

Mossing, M.C.

Assembly

CRO Repressor Insertion Mutant K56-[DGEVK]

Entity

1. CRO Repressor Insertion Mutant K56-[DGEVK] (polymer), 71 monomers, 7891.941 Da Detail

MEQRITLKDY AMRFGQTKTA KDLGVYQSAI NKAIHAGRKI FLTINADGSV YAEEVKDGEV KPFPSNKKTT A

Formula weight

7891.941 Da

Source organism

Escherichia virus Lambda

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY/ SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.5 %, Completeness (bb): 99.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	84.5 % (431 of 510)	82.1 % (357 of 435)	98.7 % (74 of 75)
Backbone	99.1 % (212 of 214)	99.3 % (144 of 145)	98.6 % (68 of 69)
Sidechain	74.0 % (219 of 296)	73.4 % (213 of 290)	100.0 % (6 of 6)
Aromatic	44.8 % (13 of 29)	44.8 % (13 of 29)
Methyl	100.0 % (38 of 38)	100.0 % (38 of 38)

1. CRO Repressor Insertion Mutant K56-[DGEVK]

MEQRITLKDY AMRFGQTKTA KDLGVYQSAI NKAIHAGRKI FLTINADGSV YAEEVKDGEV KPFPSNKKTT A

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.6

#	Name	Isotope labeling	Type	Concentration
1	CRO Repressor Insertion Mutant K56-[DGEVK]			0.0 ~ 0.0 mM
2	H2O			90 %
3	D2O			10 %

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Protein Blocks Logo

Calculated from 32 models in PDB: 2ORC, Strand ID: A Detail

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Release date

2000-03-09

Citation 1

Solution structure and dynamics of a designed monomeric variant of the lambda Cro repressor
Mossing, M.C.
Protein Sci. (1998), 7, 983-993, PubMed 9568905 , DOI 10.1002/pro.5560070416 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. CRO Repressor Insertion Mutant K56-[DGEVK], : 1 : 1 : 1 : 14 entities Detail

Interaction partners 1. CRO Repressor Insertion Mutant K56-[DGEVK], : 1 interactors Detail

Experiments performed 5 experiments Detail