BMRB: 4232

N-Domain of Troponin C from Chicken Skeletal Muscle.

Authors

Tsuda, S., Miura, A., Gagne, S.M., Spyracopoulos, L., Sykes, B.D.

Assembly

Calcium binding subunit of troponin, TnC monomer

Entity

1. Calcium binding subunit of troponin, TnC monomer (polymer), 90 monomers, 9977.024 Da Detail

ASMTDQQAEA RAFLSEEMIA EFKAAFDMFD ADGGGDISTK ELGTVMRMLG QNPTKEELDA IIEEVDEDGS GTIDFEEFLV MMVRQMKEDA

Formula weight

9977.024 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

DISGEO,SA

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 44.7 %, Completeness (bb): 72.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	44.7 % (447 of 1000)	44.5 % (230 of 517)	33.2 % (129 of 389)	93.6 % (88 of 94)
Backbone	72.3 % (389 of 538)	99.5 % (185 of 186)	44.1 % (116 of 263)	98.9 % (88 of 89)
Sidechain	13.6 % (74 of 545)	13.9 % (46 of 331)	13.4 % (28 of 209)	0.0 % (0 of 5)
Aromatic	13.3 % (8 of 60)	16.7 % (5 of 30)	10.0 % (3 of 30)
Methyl	20.9 % (18 of 86)	20.9 % (9 of 43)	20.9 % (9 of 43)

1. apoTroponin-C

ASMTDQQAEA RAFLSEEMIA EFKAAFDMFD ADGGGDISTK ELGTVMRMLG QNPTKEELDA IIEEVDEDGS GTIDFEEFLV MMVRQMKEDA

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Sample

Pressure 1 atm, Temperature 277 (±0.5) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	apoTroponin-C	[U-13C; U-15N]		1.5 mM
2	KCl			50 mM

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Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 61.5 %, Completeness (bb): 78.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	61.5 % (1231 of 2000)	69.8 % (722 of 1034)	42.0 % (327 of 778)	96.8 % (182 of 188)
Backbone	78.7 % (847 of 1076)	99.7 % (371 of 372)	56.8 % (299 of 526)	99.4 % (177 of 178)
Sidechain	44.3 % (483 of 1090)	53.3 % (353 of 662)	29.9 % (125 of 418)	50.0 % (5 of 10)
Aromatic	28.3 % (34 of 120)	51.7 % (31 of 60)	5.0 % (3 of 60)
Methyl	46.5 % (80 of 172)	60.5 % (52 of 86)	32.6 % (28 of 86)

1. apoTroponin-C

ASMTDQQAEA RAFLSEEMIA EFKAAFDMFD ADGGGDISTK ELGTVMRMLG QNPTKEELDA IIEEVDEDGS GTIDFEEFLV MMVRQMKEDA

Show sample condition

Sample

Pressure 1 atm, Temperature 277 (±0.5) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	apoTroponin-C	[U-13C; U-15N]		1.5 mM
2	KCl			50 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 1ZAC, Strand ID: A Detail

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Coupling constant

81 J values in 1 lists
Pressure 1 atm, Temperature 277 (±0.5) K, pH 6.7 (±0.2) Detail

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Pressure 1 atm, Temperature 277 (±0.5) K, pH 6.7 (±0.2)

List #1 assigned_coupling_constants_one

Release date

1999-05-24

Citation 1

Low Temperature-Induced Structural Changes in the Apo Regulatory Domain of Skeletal Muscle Troponin C
Tsuda, S., Miura, A., Gagne, S.M., Spyracopoulos, L., Sykes, B.D.
Biochemistry (1999), 38, 5693-5700, PubMed , DOI 10.1021/bi982936e

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Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. Calcium binding subunit of troponin, TnC monomer, : 1 : 15 : 7 : 299 entities Detail

Interaction partners 1. Calcium binding subunit of troponin, TnC monomer, : 3 interactors Detail

Experiments performed 3 experiments Detail