BMRB: 4241

1H and 15N Assignments of Internal xylan binding domain from XYLD

Authors

Simpson, P.J., Bolam, D.N., Cooper, A., Ciruela, A., Hazlewood, G.P., Gilbert, H.J., Williamson, M.P.

Assembly

Internal xylan binding domain from XYLD

Entity

1. Internal xylan binding domain from XYLD (polymer), 87 monomers, 8783.379 Da Detail

TGCSVTATRA EEWSDRFNVT YSVSGSSAWT VNLALNGSQT IQASWNANVT GSGSTRTVTP NGSGNTFGVT VMKNGSSTTP AATCAGS

Formula weight

8783.379 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS84:SG

Source organism

Cellulomonas fimi

Exptl. method

NMR

Refine. method

HYBRID DISTANCE GEOMETRY/ SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.8 %, Completeness (bb): 99.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	99.8 % (533 of 534)	100.0 % (436 of 436)	99.0 % (97 of 98)
Backbone	99.6 % (266 of 267)	100.0 % (182 of 182)	98.8 % (84 of 85)
Sidechain	100.0 % (267 of 267)	100.0 % (254 of 254)	100.0 % (13 of 13)
Aromatic	100.0 % (35 of 35)	100.0 % (32 of 32)	100.0 % (3 of 3)
Methyl	100.0 % (46 of 46)	100.0 % (46 of 46)

1. xylanase D

TGCSVTATRA EEWSDRFNVT YSVSGSSAWT VNLALNGSQT IQASWNANVT GSGSTRTVTP NGSGNTFGVT VMKNGSSTTP AATCAGS

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Sample

Temperature 310 (±0.3) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	xylanase D	[U-99% 15N]		1.0 mM
2	sodium_phosphate			50 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 2XBD, Strand ID: A Detail

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Release date

1999-11-28

Citation 1

The Three-Dimensional Structure of a Family IIb Xylan Binding Domain: Evidence that the Orientation of the Solvent-Exposed Tryptophans Determines the Ligand Specificity of Type II Polysaccharide Binding Domains
Simpson, P.J., Bolam, D.N., Cooper, A., Ciruela, A., Hazlewood, G.P., Gilbert, H.J., Williamson, M.P.
Structure (1999), 7, 853-864, PubMed , DOI:

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. Internal xylan binding domain from XYLD, : 1 : 2 : 2 : 12 entities Detail

Experiments performed 9 experiments Detail