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BMRB: 4249


4249
1XPA
Solution Structure of the DNA- and RPA-binding Domain of the Human Repair Factor XPA
Authors
Ikegami, T., Kuraoka, I., Saijo, M., Kodo, N., Kyogoku, Y., Morikawa, K., Tanaka, K., Shirakawa, M.
Assembly
Human NER factor XPA
Entity
1. XPA (polymer), 122 monomers, 14701.76 Da Detail

MEFDYVICEE CGKEFMDSYL MNHFDLPTCD NCRDADDKHK LITKTEAKQE YLLKDCDLEK REPPLKFIVK KNPHHSQWGD MKLYLKLQIV KRSLEVWGSQ EALEEAKEVR QENREKMKQK KF


2. ZN (non-polymer), 65.409 Da
Total weight
14767.169 Da
Max. entity weight
14701.76 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
DISTANCE GEOMETRY
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 98.4 %, Completeness: 80.9 %, Completeness (bb): 94.1 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All80.9 % (1240 of 1533)76.1 % (622 of 817)85.0 % (498 of 586)92.3 % (120 of 130)
Backbone94.1 % (681 of 724)93.8 % (228 of 243)94.8 % (344 of 363)92.4 % (109 of 118)
Sidechain72.0 % (668 of 928)68.6 % (394 of 574)76.9 % (263 of 342)91.7 % (11 of 12)
Aromatic37.7 % (46 of 122)59.0 % (36 of 61)13.6 % (8 of 59)100.0 % (2 of 2)
Methyl98.0 % (96 of 98)95.9 % (47 of 49)100.0 % (49 of 49)

1. XPA

MEFDYVICEE CGKEFMDSYL MNHFDLPTCD NCRDADDKHK LITKTEAKQE YLLKDCDLEK REPPLKFIVK KNPHHSQWGD MKLYLKLQIV KRSLEVWGSQ EALEEAKEVR QENREKMKQK KF

Show sample condition
Sample

Pressure 1 atm, Temperature 303 (±0.2) K, pH 7.3 (±0.2)


#NameIsotope labelingTypeConcentration
1Human NER factor XPA[U-13C; U-15N]1.2 mM
2zinc0.02 mM
3KCl150 mM
4DTT10 mM
5Deuterated Tris-HCl50 mM

LACS Plot; CA
Referencing offset: -0.29 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.29 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.18 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1XPA, Strand ID: A Detail

Coupling constant
89 J values in 1 lists
Pressure 1 atm, Temperature 303 (±0.2) K, pH 7.3 (±0.2) Detail
Release date
1999-08-05
Citation 1
Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA
Ikegami, T., Kuraoka, I., Saijo, M., Kodo, N., Kyogoku, Y., Morikawa, K., Tanaka, K., Shirakawa, M.
Nat. Struct. Biol. (1998), 5, 701-706, PubMed 9699634 , DOI 10.1038/1400 ,
Show more 2 citaions
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 ,
Citation 3
A short recollection on the paper entitled "A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams" by D.S. Garrett, R. Powers, A.M. Gronenborn, and G.M. Clore [J. Magn. Reson. 95 (1991) 214-220]
J. Magn. Reson. (2011), 213, 364-365, PubMed 22152356 , DOI 10.1016/j.jmr.2011.08.009 ,
Related entities 1. XPA, : 1 : 9 : 1 : 260 entities Detail
More details for 271 related entities
Interaction partners 1. XPA, : 30 interactors Detail
More details for 30 interactors identified by 10 citations
Experiments performed 1 experiments Detail
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